| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GA 657-2006 | English | 239 |
Add to Cart
|
3 days [Need to translate]
|
Detection method for ABO blood type in human body fluid stains--Hemagglutination inhibition test
| Obsolete |
GA 657-2006
|
PDF similar to GA 657-2006
Basic data | Standard ID | GA 657-2006 (GA657-2006) | | Description (Translated English) | Detection method for ABO blood type in human body fluid stains--Hemagglutination inhibition test | | Sector / Industry | Public Security (Police) Industry Standard | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 6,687 | | Date of Issue | 2006-11-03 | | Date of Implementation | 2007-03-01 | | Quoted Standard | GA 471-2004 | | Issuing agency(ies) | Ministry of Public Security | | Summary | This standard prescribes the material preparation, method of operation and results determination of the ABO blood group of the human body fluid spot by the agglutination inhibition test method in the forensic biological examination. This standard applies to the forensic examination of human liquid spot ABO blood type. | GA 657-2006: Detection method for ABO blood type in human body fluid stains--Hemagglutination inhibition test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method for ABO blood type in human body fluid stains--Hemagglutination inhibition test
ICS 13.310
A92
People's Republic of China Public Safety Industry Standard
Human body fluid spot ABO blood group detection
Agglutination inhibition test
Released on.2006-11-03
2007-03-01 implementation
Ministry of Public Security
Foreword
4.1犪 of this standard is mandatory and the rest are recommended.
Appendix A and Appendix B of this standard are normative appendices.
This standard is proposed and managed by the National Criminal Technology Standardization Committee.
This standard was drafted. Ministry of Public Security Material Certification Center, Heilongjiang Provincial Public Security Department.
Drafters of this standard. Wen Peizhong, Zhang Daan, Liu Jun, Li Xiaoping, Zhu Aimin, Li Wei, Liu Xudan, Chang Caiqin, Zhang Yinglan, Li Zhaolong, etc.
Human body fluid spot ABO blood group detection
Agglutination inhibition test
1 Scope
This standard stipulates the material preparation and operation for detecting the ABO blood type of human body fluid by agglutination inhibition test method in the examination of the physical evidence of the doctor.
Method and result determination.
This standard applies to the scientific examination of human body fluid spot ABO blood type.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GA 471-2004 anti-A, anti-B serum reagent
3 Principle
Water-soluble A, B, and H antigenic substances are present in secreted human fluid plaques, and the antigenic substance is a hapten, which can specifically
Binding to the corresponding antibody, thereby inhibiting the agglutination of the antibody and the corresponding indicator red blood cells, and agglutinating according to the antibody and the corresponding indicator red blood cells
In the case, the ABO blood type of the human body fluid spot is determined.
4 reagents and equipment
4.1 Reagents
a) Standard anti-A antibody, standard anti-B antibody reagent. Meet the requirements of GA 471-2004;
b) anti-H antibody reagent (potency should be greater than 16 times);
c) 2% to 3% of type A, type B, type O humans indicate red blood cells (see Appendix A);
d) saline.
4.2 Equipment
a) bench top centrifuge;
b) optical microscope;
c) concave glass plates, straws, test tubes, centrifuge tubes;
d) ophthalmic scissors, ophthalmology;
e) the applicator and the applicator tip;
f) Marker.
5 Method of operation
5.1 Standardization of antibodies
See Appendix B.
5.2 Inspection steps
5.2.1 Cut the body fluid spot to be sampled into a small centrifuge tube, add a small amount of saline solution (to absorb 3 drops is appropriate), and fully
Stir and soak, depending on the age of the liquid spot of the sample, soak for ten minutes to several hours.
|