| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GA 656-2006 | English | 279 |
Add to Cart
|
3 days [Need to translate]
|
Detection method for ABO blood type in human blood(stains)--Hemagglutination test, elution test
| Obsolete |
GA 656-2006
|
PDF similar to GA 656-2006
Basic data | Standard ID | GA 656-2006 (GA656-2006) | | Description (Translated English) | Detection method for ABO blood type in human blood(stains)--Hemagglutination test, elution test | | Sector / Industry | Public Security (Police) Industry Standard | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 7,790 | | Date of Issue | 2006-11-03 | | Date of Implementation | 2007-03-01 | | Quoted Standard | GA 471-2004 | | Issuing agency(ies) | Ministry of Public Security | | Summary | This standard prescribes the material preparation, operation method and result judgment of blood (scar) ABO blood type test in forensic biological examination. This standard applies to the forensic examination of human blood (scar) ABO blood type. | GA 656-2006: Detection method for ABO blood type in human blood(stains)--Hemagglutination test, elution test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method for ABO blood type in human blood(stains)--Hemagglutination test, elution test
ICS 13.310
A92
People's Republic of China Public Safety Industry Standard
Human blood (mark) ABO blood group test
Agglutination method
Released on November 11,.2006
2007-03-01 implementation
Ministry of Public Security
Foreword
4.1犪 of this standard is mandatory and the rest are recommended.
Appendix A and Appendix B of this standard are normative appendices.
This standard is proposed and managed by the National Criminal Technology Standardization Committee.
This standard was drafted. Ministry of Public Security Material Certification Center, Heilongjiang Provincial Public Security Department.
Drafters of this standard. Wen Peizhong, Zhang Daan, Liu Jun, Li Xiaoping, Zhu Aimin, Li Wei, Liu Xudan, Chang Caiqin, Zhang Yinglan, Li Zhaolong, etc.
Human blood (mark) ABO blood group test
Agglutination method
1 Scope
This standard stipulates the material preparation, operation method and result judgment of blood (mark) ABO blood group test in the physical examination of the doctor.
This standard applies to the blood (mark) ABO blood type of the forensic science tester.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GA 471-2004 anti-A, anti-B serum reagent
3 Principle
Agglutination method. A, B and H antigen substances exist on the surface of human red blood cells, under the action of anti-A antibody, anti-B antibody or anti-H antibody,
An antigen-antibody reaction occurs to cause agglutination of red blood cells. The ABO blood type can be judged based on the agglutination reaction with the corresponding antibody.
Dissociation method (protein sticking method). This method is an antigen-antibody complex formed by reacting an antigen and an antibody at a suitable temperature.
Under the condition that the antigen-antibody complex can release the antibody while maintaining the original properties of the antigen and the antibody unchanged. In the sample
In the presence of anti-A antibody or anti-B antibody, A and B blood-type substances undergo antigen-antibody reaction to form an antigen-antibody complex. When in
At 50 ° C ~ 56 ° C, the antigen-antibody complex dissociates, leaving the antibody free in the liquid, when the temperature is reduced to about 37 ° C, free
The antibody reacts with the corresponding indicator erythrocyte antigen, and agglutination can be observed under the microscope. According to the corresponding instructions
In the case of agglutination reaction, the antigen carried by the blood mark can be inferred to determine the ABO blood type.
4 Reagents and equipment
4.1 Reagents
a) Standard anti-A antibody, standard anti-B antibody reagent. Meet the requirements of GA 471-2004;
b) methanol (analytical grade);
c) saline;
d) Type A and Type B indicate red blood cell suspension (0.1% to 0.2%) (see Appendix A);
e) Egg white solution (3%) (see Appendix B).
4.2 Equipment
a) bench top centrifuge;
b) optical microscope;
c) a vacuum suction device;
d) an incubator;
e) the applicator and the applicator tip;
f) ophthalmic scissors, ophthalmology, separation needles;
g) slides, coverslips, slide holders, wash holders, humidors, white porcelain plates, capillaries;
h) Glass pen and filter paper.
|