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Detection method for ABO blood type in human hairs--Elution test
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GA 655-2006
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Basic data | Standard ID | GA 655-2006 (GA655-2006) | | Description (Translated English) | Detection method for ABO blood type in human hairs--Elution test | | Sector / Industry | Public Security (Police) Industry Standard | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 5,529 | | Date of Issue | 2006-11-03 | | Date of Implementation | 2007-03-01 | | Quoted Standard | GA 471-2004 | | Issuing agency(ies) | Ministry of Public Security | | Summary | This standard prescribes the material preparation, operation method and result judgment of ABO blood type test of human hair in forensic biological material examination. This standard is applicable to the forensic examination of human hair ABO blood type. | GA 655-2006: Detection method for ABO blood type in human hairs--Elution test---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method for ABO blood type in human hairs--Elution test
ICS 13.310
A92
People's Republic of China Public Safety Industry Standard
Human hair ABO blood group detection dissociation method
Released on November 11,.2006
2007-03-01 implementation
Ministry of Public Security
Foreword
4.1犪 of this standard is mandatory and the rest are recommended.
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Criminal Technology Standardization Committee.
This standard was drafted. Ministry of Public Security Material Certification Center, Heilongjiang Provincial Public Security Department.
Drafters of this standard. Wen Peizhong, Zhang Daan, Liu Jun, Li Xiaoping, Zhu Aimin, Li Wei, Liu Xudan, Chang Caiqin, Zhang Yinglan, Li Zhaolong, etc.
Human hair ABO blood group detection dissociation method
1 Scope
This standard stipulates the material preparation, operation method and result judgment of human hair ABO blood group test in the physical examination of the doctor.
This standard applies to the forensic science of human hair ABO blood type.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GA 471-2004 anti-A, anti-B serum reagent
3 Principle
The dissociation method is an antigen-antibody complex formed by the reaction of an antigen and an antibody, and the antigen-antibody complex can be released under certain conditions.
Antibodies are designed to maintain the original properties of antigens and antibodies. The ABO blood group substance in the hair sample is present in the medulla
In the case where an anti-A antibody or an anti-B antibody is present, an antigen-antibody reaction occurs to form an antigen-antibody complex. When at 50 ° C ~ 56 ° C
Under the condition, the antigen-antibody complex dissociates and the antibody is released into the liquid. When the temperature is lowered to about 37 ° C, the free antibody and phase
The agglutination reaction of the red blood cell antigen should be indicated, and agglomerates can be observed under the microscope. According to the corresponding indication of red blood cell agglutination
The ABO blood type can be judged according to the situation.
4 Reagents and equipment
4.1 Reagents
a) Standard anti-A antibody, standard anti-B antibody reagent. Meet the requirements of GA 471-2004;
b) Type A and Type B indicate red blood cell suspension (0.2%) (see Appendix A);
c) absolute ethanol;
d) anhydrous ether;
e) washing liquid;
f) saline.
4.2 Equipment
a) bench top centrifuge;
b) optical microscope;
c) a vacuum suction device;
d) an incubator;
e) refrigerator;
f) the applicator and the applicator tip;
g) ophthalmic scissors, ophthalmology;
h) a crimping machine or other alternative equipment or equipment;
i) concave glass plate;
j) slides, coverslips, slide holders, humidors, capillaries;
k) Marker, filter paper, cotton wool.
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