SN/T 2532-2010 English PDF

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SN/T 2532-2010: Determination of Coxsackieviruses in shellfish and water. Conventional RT-PCR and real-time RT-PCR
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2532-2010	369	Add to Cart	3 days	Determination of Coxsackieviruses in shellfish and water. Conventional RT-PCR and real-time RT-PCR	Valid

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Basic data

Standard ID: SN/T 2532-2010 (SN/T2532-2010)
Description (Translated English): Determination of Coxsackieviruses in shellfish and water. Conventional RT-PCR and real-time RT-PCR
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C53
Classification of International Standard: 07.100.30
Word Count Estimation: 14,124
Date of Issue: 2010-03-02
Date of Implementation: 2010-09-16
Quoted Standard: SN/T 1193
Regulation (derived from): National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the shellfish and water samples B2, B3, B5, A9 and A16 ordinary type Coxsackie virus by RT-PCR and real-time fluorescent RT-RCR method. This standard applies to shellfish and water samples B2, B3, B5, A9 and A16 type qualitative detection of Coxsackie virus.

SN/T 2532-2010: Determination of Coxsackieviruses in shellfish and water. Conventional RT-PCR and real-time RT-PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of Coxsackieviruses in shellfish and water.Conventional RT-PCR and real-time RT-PCR Exit inspection and quarantine industry standard book People's Republic of China Shellfish and water samples Coxsackie virus detection methods Common RT-PCR method And real-time RT-PCR. Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Appendix A of this standard is a normative appendix, Appendix B is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Xiang, Yang Jie Lin, Pan Liangwen, Huang, Zhong Shan Lu, Zhang Shuya, Lu Rong, Liu Yueming, high Qin, Liu new generations. This standard is the first release of the entry-exit inspection and quarantine industry standards. Shellfish and water samples Coxsackie virus detection methods Common RT-PCR method And real-time RT-PCR.

1 Scope

This standard specifies the shellfish and water samples B2, B3, B5, A9 and A16 Coxsackie virus common RT-PCR and real-time fluorescence method RT-PCR method. This standard applies to shellfish and water samples B2, qualitative detection B3, B5, A9 and A16 Coxsackie virus.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SN/T 1193 genetic testing laboratory technical requirements

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced. 3.2 Recombinant molecule comprising a virus-specific cDNA fragments can be detected as a virus PCR positive control.

4 Method summary

Viral RNA was extracted shellfish sample with a suitable lysis buffer (such as Tri-reagent), and in accordance with Coxsackie virus RNA3 'end Poly (A) structure, connecting with Oligo (dT) 25 magnetic beads specific adsorption of RNA was purified coxsackievirus. Viruses in water samples After enrichment, using the appropriate method for the extraction and purification of viral RNA. Use ordinary fluorescent RT-PCR or real-time RT-PCR method into Row detector. In this study, by constructing a plasmid standard molecules (plasmid molecule contains one copy of each amplified fragment), respectively, adapted to determine Five Serum Coxsackie virus detection system common RT-PCR detection limit were 50 copies, real-time fluorescent RT-PCR system, the detection limit is 2 copy.

5 Reagents

All experimental reagents were of analytical grade; unless otherwise stated, the test water is distilled or deionized water. 5.1 positive samples. Coxsackie virus, -80 ℃ refrigerator; or a plasmid containing standard molecular fragments of coxsackie virus detection purposes, -20 ℃ refrigerator. 5.2 glycine buffer. see Appendix A. 1.1. 5.3 PEG8000 solution. See Appendix A. 1.2. 5.4 lysate. Tri-reagent or other equivalent lysate (eg. TRIzol). 5.5 Oligo (dT) 25 Beads. Dynabeads-oligo (dT) 25 or equivalent products. ......

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