SN/T 2530-2010 English PDFUS$369.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2530-2010: Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR Status: Valid
Basic dataStandard ID: SN/T 2530-2010 (SN/T2530-2010)Description (Translated English): Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: C53 Classification of International Standard: 07.100.30 Word Count Estimation: 14,149 Date of Issue: 2010-03-02 Date of Implementation: 2010-09-16 Quoted Standard: SN/T 1193 Regulation (derived from): National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the shellfish, fruits and vegetables and water samples poliovirus ordinary and real-time RT-PCR and fluorescent RT-RCR method. This standard applies to shellfish, fruits and vegetables and water samples in the qualitative detection of poliovirus. SN/T 2530-2010: Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of polioviruses in shellfish, fruit, vegetable and water.Conventional RT-PCR and real-time RT-PCR Exit inspection and quarantine industry standard book People's Republic of China Shellfish, fruits and vegetables and water samples poliovirus Detection by RT-PCR and common Real-time fluorescent RT-PCR. Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordAppendix A of this standard is a normative appendix, Appendix B is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Xiang, Panliang Wen, Zhong Shan Lu, Lu Rong, Zhang Shuya, Huang, Liu Yueming, high piano. This standard is the first release of the entry-exit inspection and quarantine industry standards. Shellfish, fruits and vegetables and water samples poliovirus Detection by RT-PCR and common Real-time fluorescent RT-PCR.1 ScopeThis standard specifies the shellfish, fruits and vegetables and water samples poliovirus ordinary by RT-PCR and real-time RT-PCR method. This standard applies to qualitative detection of shellfish, fruits and vegetables and water samples poliovirus.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SN/T 1193 genetic testing laboratory technical requirements3 Terms and DefinitionsThe following terms and definitions apply to this standard. 3.1 When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced. 3.2 Recombinant molecule comprising a virus-specific cDNA fragments can be detected as a virus PCR positive control.4 Method summaryViral RNA was extracted shellfish sample with a suitable lysis buffer (such as Tri-reagent), and in accordance with poliovirus RNA3 'end Containing Poly (A) structure, connecting with Oligo (dT) 25 magnetic beads specific adsorption of poliovirus RNA was purified. Water samples The virus enrichment, using the appropriate method for the extraction and purification of viral RNA. Eluted with a suitable buffer virus sample surface of fruits and vegetables After the virus enrichment, and use the appropriate method for the extraction and purification of viral RNA. Use ordinary fluorescent RT-PCR or real-time RT-PCR Methods for testing. In this study, by constructing a plasmid standard molecules (plasmid molecule contains one copy of each amplified fragment), to determine the suitable type Ⅰ, Ⅱ type, Ⅲ type polio virus detection system common RT-PCR detection limit were 50 copies, real-time fluorescent RT-PCR detection system Measurement limit is 2 copies.5 ReagentsAll experimental reagents were of analytical grade; unless otherwise stated, the test water is distilled or deionized water. 5.1 positive samples. poliovirus, -80 ℃ refrigerator; or containing polio virus detection fragment of the plasmid standard score Son, -20 ℃ refrigerator. 5.2 glycine buffer. see A. 1.1. 5.3 PEG8000 solution. see A. 1.2. 5.4 PBS buffer. see A. 1.3. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2530-2010_English be delivered?Answer: Upon your order, we will start to translate SN/T 2530-2010_English as soon as possible, and keep you informed of the progress. 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