SN/T 2071-2008 English PDF

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SN/T 2071-2008: Identification of Candidatus liberobacter asiaticum
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2071-2008	189	Add to Cart	3 days	Identification of Candidatus liberobacter asiaticum	Valid

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Basic data

Standard ID: SN/T 2071-2008 (SN/T2071-2008)
Description (Translated English): Identification of Candidatus liberobacter asiaticum
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C53
Word Count Estimation: 7,750
Date of Issue: 2008-04-29
Date of Implementation: 2008-11-01
Regulation (derived from): Industry standard filing Notice 2008 No. 7
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the entry and exit of plant quarantine in Asian Citrus Huanglongbing pathogen quarantine and identification methods. This standard applies to the entry and exit of citrus seeds, seedlings and other propagating material of host and citrus fruits and other plant products in Asian Citrus Huanglongbing pathogen quarantine and identification.

SN/T 2071-2008: Identification of Candidatus liberobacter asiaticum

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Candidatus liberobacter asiaticum Exit inspection and quarantine industry standard book People's Republic of China Huanglong Asian citrus quarantine and identification of bacteria Posted 2008-04-29 2008-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine Animal and Plant Quarantine real The inspection. The main drafters of this standard. Li today, the Wangnian Wu, Zhouwei Chuan, water Zhu Fang, Lin Yang Wu. This standard is the first release of the entry-exit inspection and quarantine industry standards. Huanglong Asian citrus quarantine and identification of bacteria

1 Scope

This standard specifies the export plant quarantine in quarantine and identification of the Asian citrus Huanglong bacteria. This standard applies to the entry and exit of citrus seeds, seedlings and other propagation material and host citrus fruits and other plant products in the Asian citrus greening disease Quarantine and identification of bacteria. Principle 2 2.1 Asian citrus Huanglong germs taxonomic status 2.2 Identification of principle quarantine The use of polymerase chain reaction (polymerasechainreaction) technology for quarantine and identification of the Asian citrus greening disease. PCR products It was subjected to sequencing and BLAST, used to distinguish the Asian citrus Huanglong Huanglong bacteria and two other citrus bacteria.

3 Instrument

3.1 research body. 3.2 white porcelain plate. 3.3 pH meter. 3.4 PCR tubes, Eppendorf tubes (1.5mL). 3.5 pipette (1000μL adjustable, 200μL adjustable, 20μL adjustable, 2.5μL adjustable). 3.6 temperature water bath or incubator. 3.7 Analysis of electronic balance. 3.8 cryogenic speed centrifuge, low speed centrifuge. 3.9 PCR amplification. 3.10 electrophoresis. 3.11 gel imager. 3.12 cryogenic refrigerator. 3.13 Autoclave. 3.14 clean bench.

4 Reagents Drugs

4.1 50mmol/L of Tris-HCl. 4.2 10mmol/LEDTA (pH8.0). 4.3 0.2mol/L NaOH + 1% SDS. 4.4 0.5mL of sodium acetate (pH4.8). 4.5 liquid nitrogen. Ethanol 4.6 double distilled water (DDW). 4.7 primers. 4.8 10 × PCR buffer, T q Rao polymerase. ......

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