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SN/T 1896-2007 English PDF

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1896-2007799 Add to Cart 5 days Rapid enumeration of coliforms and Escherichia coli in foods. Petrifilm TM staph coliform count plate and Petrifilm TM E.coliform count plate method Obsolete

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Basic data

Standard ID: SN/T 1896-2007 (SN/T1896-2007)
Description (Translated English): Rapid enumeration of coliforms and Escherichia coli in foods. Petrifilm TM staph coliform count plate and Petrifilm TM E.coliform count plate method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C53
Classification of International Standard: 07.100.30
Word Count Estimation: 20,275
Date of Issue: 2007-05-23
Date of Implementation: 2007-12-01
Quoted Standard: SN 0169
Adopted Standard: AOAC 991.14, MOD
Regulation (derived from): Accredidation-Technology-Letter [2015] No. 247
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the coliforms and E. coli in foods Determination This standard applies to food and raw materials coliforms and E. coli counts, also applies to the surface of the health testing.

SN/T 1896-2007: Rapid enumeration of coliforms and Escherichia coli in foods. Petrifilm TM staph coliform count plate and Petrifilm TM E.coliform count plate method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Rapid enumeration of coliforms and Escherichia coli in foods.Petrifilm TM staph coliform count plate and Petrifilm TM E.coliform count plate method Exit inspection and quarantine industry standard book People's Republic of China Coliforms and E. coli in food fast counting Posted 2007-05-23 2007-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Modifications of the standard first method uses the American Society of Analytical Chemistry (AOAC) 991.14 Official Method "coliforms and E. coli in foods Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Entry-Exit Inspection and Quarantine of Zhejiang People's Republic of China Bureau, People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Inner Mongolia, People's Republic of China, People's Republic of China State CIQ Shandong, Dalian Kai Yuan Science and Technology Development Co., 3M China Limited. The main drafters of this standard. Luxing An, Shi Weiliang, Zhao, Zhang Hongwei, Kit Cheng, Liu high school, Wang Jinling, Wu Gang, Ray quality Wen, Liu Ran, Zhou Zhenya, Lu Su soared. This standard is the first release of the entry-exit inspection and quarantine industry standards. Coliforms and E. coli in food fast counting

1 Scope

This standard specifies the determination of coliforms and E. coli in food (PetrifilmTM1) test piece method). This standard applies to food and feed coliforms and E. coli counts, but also for surface hygiene test. 1) for the US company 3M product trade mark.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SN 0169 export food coliform, fecal coliform and E. coli test methods Principle 3 3.1 PetrifilmTM coliform test piece (PetrifilmTMColiformCountPlate) system is a good culture medium prepared in advance, Containing VRB (VioletRedBile) medium, cold water soluble gel and triphenyl tetrazolium chloride (TTC) indicator can be enhanced colony counting effect. The film covering the surface, the gas can be trapped coliforms ferment lactose produce. After completion of the culture surrounding the red dot count with bubbles Coliform colonies. The prepared medium system containing VRB medium, cold water soluble gel and glucosidase indicator, can enhance the effect of colony counts. E. coli colonies around. The film covering the surface, the gas can be trapped coliforms ferment lactose produce. After incubation with the blue dot count It is the number of E. coli colonies bubbles, red and blue with bubbles with the bubble point of the colonies and coliform count.

4 Equipment and Materials

4.1 incubator. 36 ℃ ± 1 ℃. 4.2 homogenizer (rotary knife or slap-style) or equivalent device. 4.3 pH meter or pH paper precision. 4.4 PetrifilmTM test piece platen. 4.5 magnifier or (and) colony counter or automatic interpretation PetrifilmTM instrument.

5 medium and reagents

5.1 sterile saline. Weigh 8.5g of sodium chloride dissolved in 1000mL of distilled water, 121 ℃ autoclave 15min. 5.2 1mol/L sodium hydroxide (NaOH). Weigh 40g of sodium hydroxide (NaOH) was dissolved in 1000mL of distilled water. 5.3 1mol/L hydrochloric acid (HCl). Pipette concentrated hydrochloric acid 90mL, diluted with distilled water to 1000mL. 5.4 PetrifilmTM coliform test piece. 5.5 PetrifilmTM E. coli/coliform test piece.
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