SN/T 1135.3-2016 English PDF

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SN/T 1135.3-2016: Detection and identification of Potato mop-top virus
Status: Valid

SN/T 1135.3: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1135.3-2016	329	Add to Cart	3 days	Detection and identification of Potato mop-top virus	Valid
SN/T 1135.3-2003	599	Add to Cart	3 days	Quarantine methods for identification of potato mop-top Pomovirus	Obsolete

Similar standards

NY/T 2288   SN/T 1204   NY 39   SN/T 1135.8   SN/T 1135.2   SN/T 1135.11   

Basic data

Standard ID: SN/T 1135.3-2016 (SN/T1135.3-2016)
Description (Translated English): Detection and identification of Potato mop-top virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,178
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Older Standard (superseded by this standard): SN/T 1135.3-2003
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 1135.3-2016: Detection and identification of Potato mop-top virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Potato mop-top virus People's Republic of China Exit Inspection and Quarantine standards Replacing SN/T 1135.3-2003 Potato top broom virus quarantine and identification method 2016-12-12 released 2017-07-01 implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 1135 series of standards is divided into 13 parts. --- Part 1. Potato disease detection and identification of cancer; --- Part 2. Potato yellow dwarf virus quarantine and identification methods; --- Part 3. Potato top broom virus quarantine and identification methods; --- Part 4. Potato powdery mildew quarantine and identification methods; --- Part 5. Potato ringworm bacteria quarantine and identification methods; --- Part 6. Potato virus pathogen quarantine and identification methods; --- Part 7. Potato virus A quarantine and identification methods; --- Part 8. Potato asymptomatic bacteria quarantine and identification methods; --- Part 9. Potato bacterial wilt quarantine and identification methods; --- Part 10. Potato virus V quarantine and identification methods; --- Part 11. Potato pathogens quarantine and identification methods; --- Part 12. Potato M virus quarantine and identification methods; --- Part 13. Potato virus Y quarantine and identification methods. This part of the SN/T 1135 Part 3. This section drafted in accordance with GB/T 1.1-2009 given rules. This Part replaces SN/T 1135.3-2003 "potato broom top virus quarantine and identification methods." This section compared with the SN/T 1135.3-2003, the main technical differences are as follows. --- Removed "Introduction"; --- Removed the "Terms and definitions" chapter; --- Added "potato broom top virus basic information" chapter; --- Removed "on-site quarantine and sampling" chapter; --- Added "test method" section; --- Modify the RT-PCR detection method. Please note that some of this document may be patentable. The publication structure of this document does not bear the responsibility of identifying these patents. This part of the national certification and accreditation supervision and management committee proposed and centralized. This part of the drafting unit. China Inspection and Quarantine Science Institute. The main drafters of this section. Li Mingfu, Zhang Yongjiang, Ding Xiaolan, Xiang Ning, Li Guifen, Wei Meisheng, Chen Zhinan, Huang Wensheng, Zhang Chengliang. This standard replaces the standards previously issued as. --- SN/T 1135.3-2003. Potato top broom virus quarantine and identification method

1 Scope

This section provides the basic principles and methods of potato broom top virus quarantine and identification. This section applies to all entry seed, potato products, tissue culture seedlings and virus-free potato germplasm in the potato broom top virus quarantine test Field investigation of diseases caused by potato broom top virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 1840 plant virus immune electron microscopy detection method SN/T 2122 Quarantine sampling of entry and exit plants and plant products

3 potato top broom virus basic information

Chinese name. Potato top broom virus Scientific name .Patatomop-topvirus Abbreviation. PMTV Taxonomic status Virgaviridae Potato, genus Pomovirus. See Appendix A for additional information on potato broom top virus.

4 method principle

The serological characteristics, molecular biological characteristics and biological characteristics of potato broom top virus are the main basis for quarantine and identification. 5 equipment, appliances and reagents 5.1 Equipment Electronic analytical balance (0.0001g), small centrifuge, desktop refrigerated centrifuge, constant temperature water bath, microplate reader, general PCR machine, electrophoresis System, pH meter, gel imaging system, 4 ° C refrigerator, ultra clean bench, ultra low temperature refrigerator at -80 ° C, autoclave, ice maker, vortex oscillation Microwave oven, electron transmission microscope and the like. 5.2 appliances Adjustable pipette (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL, 5000 μL) and corresponding RNase-free tip, RNase-free centrifuge tubes, PCR tubes, mortars, sample bags, labels and more. 5.3 Reagents Except for special instructions, all experimental reagents are of analytical grade. ......

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