SN/T 1135.2-2016 English PDF

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SN/T 1135.2-2016: Detection and identification of Potato yellow dwarf virus
Status: Valid

SN/T 1135.2: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1135.2-2016	329	Add to Cart	3 days	Detection and identification of Potato yellow dwarf virus	Valid
SN/T 1135.2-2003	679	Add to Cart	3 days	Quarantine methods for identification of potato yellow dwarf Nucleorhabdovirus	Obsolete

Similar standards

NY/T 2288   SN/T 1204   NY 39   SN/T 1135.8   SN/T 1135.3   SN/T 1135.11   

Basic data

Standard ID: SN/T 1135.2-2016 (SN/T1135.2-2016)
Description (Translated English): Detection and identification of Potato yellow dwarf virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,120
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Older Standard (superseded by this standard): SN/T 1135.2-2003
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 1135.2-2016: Detection and identification of Potato yellow dwarf virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Potato yellow dwarf virus China's entry-exit inspection and quarantine industry standards Replacing SN/T 1135.2-2003 Potato yellowing dwarf virus quarantine and identification method Published on.2016-12-12 2017-07-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

The SN/T 1135 series of standards is divided into 13 parts. --- Part 1. Quarantine and identification methods for potato cancer; --- Part 2. Quarantine and identification methods for potato yellowing dwarf virus; --- Part 3. Quarantine and identification methods for potato vetch virus; --- Part 4. Quarantine and identification methods for potato smut powder; --- Part 5. Quarantine and identification methods for potato ring rot bacteria; --- Part 6. Quarantine and identification methods for potato rot rot. ---Part 7. Potato A virus quarantine and identification method; --- Part 8. Quarantine and identification methods for potato gangrene pathogens; ---Part 9. Quarantine and identification methods for potato bacterial wilt; --- Part 10. Potato V virus quarantine and identification methods; --- Part 11. Quarantine and identification methods for potato leaf spot pathogens; --- Part 12. Quarantine and identification methods for potato M virus; --- Part 13. Quarantine and identification methods for potato virus Y. This section is Part 2 of SN/T 1135. This section was drafted in accordance with the rules given in GB/T 1.1-2009. This section replaces SN/T 1135.2-2003 "Potato yellow dwarf virus quarantine and identification methods." This section compared with SN/T 1135.2-2003, the main technical differences are as follows. --- Deleted the "terms and definitions" chapter; --- Added chapter "Potato Yellow Dwarf Virus Basic Information"; --- Deleted the "Spot Quarantine and Sampling" section; --- Added "detection method" section; --- Increased RT-PCR detection method. Please note that some of the contents of this document may involve patents. The publication structure of this document does not assume responsibility for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Regulatory Commission. This section was drafted by. China Academy of Inspection and Quarantine. The main drafters of this section. Li Mingfu, Zhang Yongjiang, Ding Xiaolan, Liu Zhongmei, Zhao Zhu, Wang Xiufen, Li Guifen, Chen Yanfang, Zeng Qingcai, Liu Shanbin. The previous versions of the standards replaced by this standard are. ---SN/T 1135.2-2003. Potato yellowing dwarf virus quarantine and identification method

1 Scope

This part of SN/T 1135 specifies the basic principles and methods for quarantine and identification of potato yellowing dwarf virus. This section applies to the inspection of potato yellowing dwarf virus in potato germplasm such as all incoming seed potatoes, commodity potatoes, tissue culture seedlings, and virus-free seedlings. Disease identification and field investigations caused by potato yellowing dwarf virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. SN/T 1840 Plant Virus Immunoelectron Microscopy Assay SN/T 2122 Quarantine sampling of entry and exit plants and plant products

3 Potato yellowing dwarf virus basic information

Chinese name. Potato yellowing dwarf virus Scientific name. Potatoyellowdwarfvirus Abbreviation. PYDV Taxonomic status. Rhabdoviridae, Nucleorhabdovirus. See Appendix A for additional information on potato yellowing dwarf virus.

4 Methodology

The serological, molecular biological and biological characteristics of potato yellowing dwarf virus are the main basis for quarantine and identification. 5 Apparatus, Equipment, and Reagents 5.1 Instruments and Equipment Electronic analytical balance (0.0001g), small centrifuge, bench-top refrigerated centrifuge, constant-temperature water bath, microplate reader, ordinary PCR, electrophoresis System, pH meter, gel imaging system, 4 °C refrigerator, clean bench, -80 °C ultra-low temperature refrigerator, autoclave, ice maker, vortex Devices, microwave ovens, electron transmission microscopes, etc. 5.2 Appliances Adjustable micropipettes (2.5μL, 10μL, 20μL, 100μL,.200μL, 1000μL, 5000μL) and corresponding RNase-free Tips, RNase-free centrifuge tubes, PCR tubes, mortars, sample bags, labels, etc. 5.3 Reagents Except for special instructions, all experimental reagents were of analytical grade. ......

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