SN/T 1135.11-2013 English PDF

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SN/T 1135.11-2013: Detection and identification of Polyscytalum pustulans (M.N.Owen et Wakef.)M.B.Ellis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1135.11-2013	319	Add to Cart	3 days	Detection and identification of Polyscytalum pustulans (M.N.Owen et Wakef.)M.B.Ellis	Valid

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NY/T 2288   SN/T 1204   NY 39   SN/T 1135.8   SN/T 1135.3   SN/T 1135.2   

Basic data

Standard ID: SN/T 1135.11-2013 (SN/T1135.11-2013)
Description (Translated English): Detection and identification of Polyscytalum pustulans (M.N.Owen et Wakef.)M.B.Ellis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020
Word Count Estimation: 12,128
Regulation (derived from): National quality recognition (2013) 569
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the potato skin spot quarantine pathogen identification methods. This standard applies to quarantine and identification of the potato tuber potato skins leaf spot pathogen.

SN/T 1135.11-2013: Detection and identification of Polyscytalum pustulans (M.N.Owen et Wakef.)M.B.Ellis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Polyscytalum pustulans (MNOwen et Wakef.) MBEllis People's Republic of China Entry-Exit Inspection and Quarantine Standards Potato skin patch quarantine and identification of bacteria DetectionandidentificationofPolyscytalumpustulans (MNOwenetWakef.) MBElis Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This section drafted in accordance with GB/T 1.1-2009 given rules. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section drafted by. China Inspection and Quarantine Science Research Institute. Drafters of this section. Wu Shan goods, Du Hongzhong, Yan Jin, Zhang Qiue. Potato skin patch quarantine and identification of bacteria

1 Scope

SN/T 1135 This section provides the quarantine and identification of potato peel leaf spot pathogen. This section applies to quarantine and identification of potato tubers potato peel leaf spot pathogen.

2 Basic information of germs

Chinese name. potato skin patch germs English name. Skinspotofpotato Scientific name. Polyscytalumpustulans (MNOwenetWakef.) MBElis Synonym. OosporapustulansM.N.Owen Taxonomic position. Fungi (Fungi), mitosis fungal spores (Mitosporicfungi), snake Mortierella (Polyscytalum). Transmission. pathogen primary infection source is a tuber, spores by rain, irrigation close spread by seed or remote tape Soil disease spread. For additional information potato peel leaf spot pathogen in Appendix A.

3 PRINCIPLE OF THE METHOD

Potato skin patch pathogen morphology and molecular characterization of identification basis.

4 apparatus and appliances

4.1 Instrument Microscope (with measurements), stereo microscope, clean benches, biological incubator, scales, autoclaves, desktop high-speed centrifugation Machine, pH meter, water bath, real-time PCR amplification. 4.2 Appliances Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank.

5 Reagents and culture medium

5.1 Reagents Glucose, agar powder, liquid nitrogen, Tris-HCl, MgCl2, HCl, EDTA, NaOH, NaOAC, KCl, Tris, CTAB, TAE, no Aqueous ethanol (Ethanol), SYBRGreenⅠ nucleic acid dyes, chloroform (Chloroform), isopropyl alcohol (Isopropanol), iso-amyl alcohol (Isoamyl alcohol), proteinase K, TaqDNA polymerase, dNTP, universal primers ITS5 and ITS4, real-time PCR primers PpustF1, PpustR2 and probe PpustPr1. ......

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