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Inspection of grain and oils - Detection of aflatoxin B1 in cereals - Superconducting quantum dot immunofluorescence method
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Basic data
| Standard ID | LS/T 6149-2024 (LS/T6149-2024) |
| Description (Translated English) | Inspection of grain and oils - Detection of aflatoxin B1 in cereals - Superconducting quantum dot immunofluorescence method |
| Sector / Industry | Food & Beverage Industry Standard (Recommended) |
| Classification of Chinese Standard | B20 |
| Classification of International Standard | 67.060 |
| Word Count Estimation | 10,132 |
| Date of Issue | 2024-10-01 |
| Date of Implementation | 2025-04-01 |
| Issuing agency(ies) | National Grain and Material Reserves Administration |
LST6149-2024: Inspection of grain and oils - Detection of aflatoxin B1 in cereals - Superconducting quantum dot immunofluorescence method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.060
CCSB20
Grain Industry Standard of the People's Republic of China
Grain and oil inspection - Determination of aflatoxin B1 in cereals
Superconducting dot immunofluorescence rapid quantitative method
Released on 2024-10-01
2025-04-01 Implementation
The State Administration of Grain and Material Reserves released
Published by China Standards Press
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Cereals and Oils Standardization (SAC/TC270).
This document was drafted by. Hunan Grain and Oil Product Quality Monitoring Center, Shenzhen Saitainuo Biotechnology Co., Ltd., Beijing Food Inspection
Institute of Food Safety (Beijing Food Safety Monitoring and Risk Assessment Center), Liaoning Provincial Grain and Material Reserve Affairs Service Center, Anhui Provincial Grain and Oil Production and
Food Quality Supervision and Inspection Station, Jiangxi Provincial Inspection, Testing and Certification Institute Food Inspection and Testing Institute, Guangdong Provincial Food Science Research Institute Co., Ltd.,
Sichuan Provincial Grain Quality Monitoring Center.
The main drafters of this document are. Xu Yanxia, Chen Quling, Ni Xiaoying, Zhang Xiaoyan, Liu Hongmei, Ma Zhi, Qiao Lina, Shen Na, Hu Bin, Yang Jing,
Peng Zhibing, Chen Wei, Liu Xiangzhao, Huang Wei, Mei Guang, Hong Ling, Han Yuhui, Li Xinyang.
Grain and oil inspection - Determination of aflatoxin B1 in cereals
Superconducting dot immunofluorescence rapid quantitative method
1 Scope
This document describes a method for the rapid quantitative determination of aflatoxin B1 in cereals by superconducting dot immunofluorescence, and specifies the reagents,
Instruments and materials, sample preparation, sample measurement, result presentation and repeatability.
This document is applicable to the rapid quantitative detection of aflatoxin B1 in wheat, rice and corn.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 5491 Sampling and subsampling methods for grain and oilseed inspection
GB/T 6682 Specifications and test methods for water used in analytical laboratories
LS/T 6402 General principles for the suitability verification and result evaluation of grain and oil inspection equipment and methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Quantum dot atomic luminescent materials coated with super semiconductors.
4 Principle
The aflatoxin B1 antigen in the sample and the aflatoxin B1 antigen fixed on the detection line T line and the superconducting amount point labeled aflatoxin
The fluorescence signal read by the fluorescence analyzer is inversely proportional to the aflatoxin B1 content in the sample.
The aflatoxin B1 content in the sample can be automatically calculated by setting the curve.
5 Reagents
Unless otherwise specified, all reagents used are of analytical grade and the water used in the laboratory should meet the requirements for grade 3 water in GB/T 6682.
5.1 Disodium hydrogen phosphate (Na2HPO4).
5.2 Sodium dihydrogen phosphate (NaH2PO4).
5.3 Potassium chloride (KCl).
5.4 Tween 80 (C32H60O10).
5.5 Methanol (CH3OH).
5.6 Dilution buffer. weigh 3.5 g of disodium hydrogen phosphate (5.1), 1.5 g of sodium dihydrogen phosphate (5.2), and 0.2 g of potassium chloride (5.3).
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