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Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships
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Basic data
| Standard ID | GB/T 28229-2011 (GB/T28229-2011) |
| Description (Translated English) | Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | C51 |
| Classification of International Standard | 07.100.20 |
| Word Count Estimation | 11,113 |
| Date of Issue | 2011-12-30 |
| Date of Implementation | 2012-05-01 |
| Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the entry and exit of ships' ballast water and diarrhea Escherichia coli (diarrheagenic Escherichia coli) testing procedures and methods. This standard applies to the entry-exit ship ballast water diarrhea detection of Escherichia coli. |
GB/T 28229-2011: Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships
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Detection of diarrheagenic Escherichia coli in ballast water of entry-exit ships
ICS 07.100.20
C51
National Standards of People's Republic of China
Entry and exit of ships ballast water cause diarrhea E. coli
Testing method
Issued on. 2011-12-30
2012-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The revised standard used by the US Food and Drug Administration (FDA) "bacteriological test manual. diarrhea caused by Escherichia coli" (2009
July) (BAM. DiarrheagenicEscherichiacoli, July2009).
The main difference between this standard and the FDA approach are.
--- Deletion of some virulence factor detecting step;
--- Delete real-time PCR screening step;
--- Culture temperature from 35 ℃ ± 0.5 ℃ revised to 36 ℃ ± 1 ℃.
This standard was proposed by the State Administration of Quality Supervision, Inspection and Quarantine.
The standard by the China Academy of Inspection and Quarantine.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Qinhuangdao Exit Inspection and Quarantine, the Chinese people
Exit Inspection and Quarantine of the Republic of Guangzhou.
The main drafters of this standard. Wang Lin, Zhang Shun, normally forest, Lijun Cheng, Zhang Yue, Li there, Li Xin, Nie Weizhong, Liao Ruyan, Chen Chuntian,
Chi Ying, Liu Endong, Han Hui.
Entry and exit of ships ballast water cause diarrhea E. coli
Testing method
1 Scope
This standard specifies the entry and exit of ships ballast water cause diarrhea Escherichia coli (diarrheagenicEscherichiacoli) testing procedures
And methods.
This standard applies to the entry and exit of ships' ballast water cause diarrhea E. coli detected.
2 Definitions
The following terms and definitions apply to this document.
2.1
Diarrhea caused by Escherichia coli diarrheagenicEscherichiacoli
Part of the genus Escherichia virulent, can cause diarrhea, collectively known strains of human disease, generally including enteropathogenic
Escherichia coli (EPEC), enterohemorrhagic Escherichia coli (EHEC), intestinal invasive Escherichia coli (EIEC ), enterotoxigenic large
Escherichia coli (ETEC) and the like.
3 equipment and materials
3.1 Refrigerator. 2 ℃ ~ 8 ℃.
3.2 incubator. 36 ℃ ± 1 ℃, 44 ℃ ± 1 ℃.
3.3 filter collection device bacteria.
3.4 UV lamp. wavelength 365nm.
3.5 sterile conical flask. 500mL.
3.6 sterile pipette. 25mL, accurate to 0.1mL.
3.7 Pipette. 0.1mL, accurate to 0.01mL.
3.8 inoculating loop.
3.9 inoculating needle.
3.10 sterile Petri dish. 90mm.
4 media and reagents 1)
1) recommends using a commercial medium and biochemical reaction tube to ensure stable quality, biochemical reactions can also be used to identify microorganisms automatic biochemical equipment to complete.
4.1 brain heart infusion broth (BHI).
4.2 Double tryptone phosphate broth (dTP). See A.1.
4.3 EHEC enrichment broth (EEB). See A.2.
4.4 eosin methylene blue agar (EMB). See A.3.
4.5 MacConkey agar (MAC). see A.4.
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