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Detection of Listeria monocytogenes in ballast water of entry-exit ships
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Basic data
| Standard ID | GB/T 28228-2011 (GB/T28228-2011) |
| Description (Translated English) | Detection of Listeria monocytogenes in ballast water of entry-exit ships |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | C51 |
| Classification of International Standard | 07.100.20 |
| Word Count Estimation | 10,197 |
| Date of Issue | 2011-12-30 |
| Date of Implementation | 2012-05-01 |
| Adopted Standard | FDA BAM-2003, MOD |
| Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the entry and exit of ships' ballast water and Listeria monocytogenes bacteria (Listeria monocytogenes) testing procedures and methods. This standard applies to the entry and exit of Ships' Ballast Water Listeria monocytogenes bacteria testing. |
GB/T 28228-2011: Detection of Listeria monocytogenes in ballast water of entry-exit ships
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Detection of Listeria monocytogenes in ballast water of entry-exit ships
ICS 07.100.20
C51
National Standards of People's Republic of China
Entry and exit of ships ballast water monocytes
Test method of Listeria
Issued on. 2011-12-30
2012-05-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The revised standard used by the US Food and Drug Administration (FDA) "bacteriological test manual. Listeria monocytogenes bacteria in check
Measure and count "(January 2003) (BAM. DetectionandenumerationofListeriamonocytogenes, January2003).
The main difference between this standard and the FDA approach are.
--- Increased screening step;
--- Clear the counting step;
--- Incubator temperature from 35 ℃ revised to 36 ℃ ± 1 ℃.
This standard was proposed by the State Administration of Quality Supervision, Inspection and Quarantine.
The standard by the China Academy of Inspection and Quarantine.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Qinhuangdao Exit Inspection and Quarantine, the Chinese people
Republic SZCIQ.
The main drafters of this standard. Shun together, Wang Lin, Pangqiu Yan, Zhang Yue, Li has, Lijun Cheng, Li Xin, Nie Weizhong, Chen Chuntian, FORCES,
Chi Ying, Zhao Bin, Han Hui.
Entry and exit of ships ballast water monocytes
Test method of Listeria
1 Scope
This standard specifies the entry and exit of ships' ballast water monocytogenes Listeria (Listeriamonocytogenes) detection process
Order and method.
This standard applies to detect the entry and exit of ships ballast water Listeria monocytogenes bacteria.
2 Equipment and Materials
2.1 Refrigerator. 2 ℃ ~ 8 ℃.
2.2 incubator. 30 ℃ ± 1 ℃, 36 ℃ ± 1 ℃.
2.3 Microscope. 10 × ~ 100 ×.
2.4 Sterile pipette or a sterile graduated cylinder. 1mL, 25mL, accurate to 0.1mL.
2.5 conical flask. 500mL.
2.6 sterile Petri dish. diameter of 90mm.
2.7 sterile tube. 16mm × 125mm.
2.8 inoculating loop.
2.9 inoculating needle.
2.10 Staphylococcus aureus (ATCC25923).
2.11 Rhodococcus equi (Rhodococcusequi).
2.12 Iraq's Listeria.
2.13 monocytogenes Listeria.
Listeria innocua 2.14.
3 media and reagents
3.1 Listeria enrichment broth buffer (BLEB). See A.1.
3.2 PALCAM Agar. see A.2.
3.3 ALOA chromogenic medium 1).
3.4 containing 0.6% yeast extract of trypticase soy agar (TSAye). See A.3.
3.5 SIM medium power. see A.4.
3.6 5% sheep blood agar. see A.5.
3.7 Gram staining solution.
3.8 3% hydrogen peroxide solution. Test for catalase.
3.9 biochemical reaction tube 2). rhamnose, xylose, glucose, maltose, mannitol, MR/VP test.
1) provided by the French company AES product trade name, this information is given for convenience only user of this standard does not imply endorsement of the product, such as
Other fruit products have the same effect, you can use these equivalent products.
2) recommend the use of commercially available biochemical reaction tube to ensure stable quality, can also be used biochemical automated microbial identification system completed.
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