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| GB/T 29582-2013 | English | 150 |
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Detection and identification of peanut stunt virus
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GB/T 29582-2013
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Basic data | Standard ID | GB/T 29582-2013 (GB/T29582-2013) | | Description (Translated English) | Detection and identification of peanut stunt virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 13,153 | | Quoted Standard | SN/T 1840; SN/T 2122 | | Regulation (derived from) | National Standards Bulletin 2013 No. 10 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies: peanut stunt virus quarantine and identification methods. This standard applies to live plant material, including vegetative propagation material, tissue culture, seeds and seedlings of peanut stunt virus detection. | GB/T 29582-2013: Detection and identification of peanut stunt virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of peanut stunt virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Identification method of peanut dwarf virus
2013-07-19 release
2013-12-06 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271).
The drafting unit of this standard. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Entry-Exit Inspection and Quarantine of the People's Republic of China
Bureau of the People's Republic of China Xiamen Entry - Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Yu Cui, Yang Cuiyun, India Liping, Zheng Yun, Liao Furong, Zheng Jianzhong, Yi Jianping.
Identification method of peanut dwarf virus
1 Scope
This standard specifies the method of identification of peanut dwarf virus.
This standard applies to the detection of peanut dwarf virus in living plant materials, including asexual propagation material, tissue culture seedlings, seeds and seedlings.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
SN/T 1840 Plant virus immunoelectron microscopy
SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products
3 peanut dwarf virus basic information
Chinese name. peanut dwarf virus.
English name. Peanutstuntvirus.
Synonyms. groundnutstuntvirus; peanutstuntcucumovirus; robinia mosaicvirus; cloverblotch
virus; peanutcommonmosaicvirus
Abbreviations. PSV.
Bromoviridae, Cucumber mosaic virus is Cucumovirus.
See Appendix A for additional information on peanut dwarf virus.
4 Principle of the method
Using double antibody sandwich enzyme-linked immunosorbent assay based on antigen-antibody response (doubleantibodys and wichenzyme-linked
immunosorbentassay, DAS-ELISA), reverse transcription and in vitro DNA synthesis techniques in reverse transcription polymerase chain reaction
(reverse transcriptase polymerase chain reaction, RT-PCR).
5 Instruments, facilities, utensils and reagents
5.1 Instruments
PCR instrument, clean bench, electronic balance (0.001g), electrophoresis, electrophoresis tank, gel imaging system, electron transmission microscope, water bath
Pot, high-speed refrigerated centrifuge, -80 ℃ ultra-low temperature refrigerator, autoclave, ice maker, microwave oven, scroll oscillator.
5.2 facilities
Isolated greenhouse (10 ℃ ~ 30 ℃).
5.3 appliances
(2 μL, 10 μL, 100 μL,.200 μL, 1000 μL, 5000 μL) and the corresponding RNase-free tip, no
RNase centrifuge tube, PCR tube and mortar.
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