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GB 29989-2013 PDF in English
GB 29989-2013 (GB29989-2013) PDF English
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GB 29989-2013
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Determination of L-carnitine in infant foods and dairy products
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GB 29989-2013
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GB 29989-2013: PDF in English
GB 29989-2013 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard - Determination of Levocarnitine in Foods and Milk Products for Infants and Young Children ISSUED ON. NOVEMBER 29, 2013 IMPLEMENTED ON. JUNE 1, 2014 Issued by. National Health and Family Planning Commission of the People's Republic of China Table of Contents 1 Scope ... 3 2 Principle ... 3 3 Reagents and Materials ... 3 4 Apparatuses and devices ... 5 5 Analytical Procedures ... 5 6 Expression of analytical result ... 6 7 Precision ... 6 8 Other ... 6 National Food Safety Standard - Determination of Levocarnitine in Foods and Milk Products for Infants and Young Children 1 Scope This Standard specifies the method for determination of levocarnitine in foods and milk products for infants and young children. This Standard is applicable to the determination of levocarnitine in foods and milk products for infants and young children. 2 Principle Extract the specimen with water; use perchloric acid to precipitate the protein; then filtrate it. After the filtrate is gone through alkaline saponification, the combined-state levocarnitine in solution is dissociated. Under the catalysis of carnitine acetyl transferase, levocarnitine reacts with acetyl coenzyme A to generate acetylcarnitine and free coenzyme A. Free coenzyme A reacts with 2-nitrobenzoic acid to generate yellow substance; the color of which is proportional to the content of free coenzyme A. As the free coenzyme A and levocarnitine are of same mole reaction, the content of levocarnitine in specimen may be calculated indirectly. 3 Reagents and Materials Note. Unless otherwise specified, all reagents adopted in this method are analytical reagents and the water is Grade 3 specified in GB/T 6682. 3.1 Reagents 3.1.1 Perchloric acid (HClO4). 3.1.2 Sodium hydroxide (NaOH). 3.1.3 Potassium hydroxide (KOH). 3.1.4 2-nitrobenzoic acid (C14H8N2O8S2). 3.1.5 N-2-hydroxyethyl piperazine-N-2-ethanesulfonic acid (C8H18N2O4S). 6 Expression of analytical result The content X of levocarnitine in specimen is expressed by mass fraction mg/100g and calculated according to Formula (1). cX m V ... (1) Where, X - the content of levocarnitine in specimen, mg/100g; c - the concentration of specimen treatment solution obtained through standard curve, μg/mL; V - the filtrate volume, mL; m - the specimen mass, g. The result is expressed as the arithmetic mean of two independent determination results under repeatability conditions, accurate to one decimal place. 7 Precision The absolute deviation of two independent determination results under repeatability conditions shall not exceed 10% of arithmetic mean. 8 Other Method detection limit is 0.6mg/100g; quantitation limit is 2mg/100g.
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Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.