GB 1886.174-2024 PDF English
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 1886.174-2024 | English | 320 |
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National Food Safety Standard - Food additives - Enzyme Preparations for Food Industry
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| GB 1886.174-2016 | English | 230 |
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National food safety standard - Food additives - Enzyme preparation for food industry
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GB 1886.174-2024: National Food Safety Standard - Food additives - Enzyme Preparations for Food Industry
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB1886.174-2024
GB
NATIONAL STANDARD OF THE
PEOPLE'S REPUBLIC OF CHINA
National food safety standard - Food additives - Enzyme
preparations for food industry
Issued on. FEBRUARY 8, 2024
Implemented on. AUGUST 8, 2024
Issued by. National Health Commission of the People's Republic of China;
State Administration for Market Regulation.
Table of Contents
Foreword... 3
1 Scope... 4
2 Terms and definitions... 4
3 Product classification... 5
4 Technical requirements... 5
Appendix A List of excipients for enzyme preparations... 7
Appendix B Reference method for determination of enzyme activity of some enzyme
preparations... 10
B.1 Reference method for determination of enzyme activity of some enzyme preparations
... 10
B.2 Determination of α-acetolactate decarboxylase activity... 11
B.3 Determination of pectinase activity... 19
B.4 Determination of glucoamylase (amyloglucosidase) activity... 22
B.5 Determination of transglucosidase activity... 25
Foreword
This standard replaces GB 1886.174-2016 National food safety standard - Food
additives - Enzyme preparations for food industry.
Compared with GB 1886.174-2016, the main changes in this standard are as follows.
-- The terms and definitions of "excipients for enzyme preparations", "immobilized
enzyme preparations" and "immobilized carrier" are added;
-- The technical requirements for excipients for enzyme preparations and
immobilized carriers are added;
-- The indicator requirements for Escherichia coli are modified;
-- Appendix requirements for the list of excipients for enzyme preparations are
added;
-- Some reference methods for enzyme activity determination in the appendix are
added.
National food safety standard - Food additives - Enzyme
preparations for food industry
1 Scope
This standard applies to enzyme preparations for the food industry that are allowed to
be used in National food safety standard - Standards for uses of food additives (GB
2760) and related announcements.
2 Terms and definitions
2.1 enzyme preparations for the food industry
A preparation product with special catalytic activity and used in the food industry,
which is directly extracted from edible or non-edible parts of animals or plants, or
fermented and extracted from microorganisms (including but not limited to bacteria,
actinomycetes, and fungal strains) obtained through traditional selection or genetic
recombination technology, or prepared through further purification, formulation and
other processes (it may contain one or more active enzyme components).
2.2 enzyme activity
An indicator of the activity of an enzyme to catalyze a specific reaction under certain
conditions.
2.3 antibacterial activity
Properties that inhibit or kill microorganisms.
2.6 immobilized carrier
The material that plays the role of immobilizing enzymes in immobilized enzyme
preparations.
3 Product classification
According to product form, it is divided into solid dosage forms (including immobilized
dosage forms) and liquid dosage forms.
4 Technical requirements
4.1 Requirements for raw materials and excipients
4.1.1 The raw materials and excipients used to produce enzyme preparations shall meet
relevant requirements and shall not produce residual contamination harmful to health
in the final food under the specified conditions of use.
4.1.5 For the activity preservation, circulation and storage, and standardized use of
enzyme preparation products, necessary excipients are allowed to be added to
commercial enzyme preparations. Excipients for enzyme preparations shall not play a
functional role in the final food. They are generally used in appropriate amounts
according to production needs, and the varieties and amounts used shall be reduced as
much as possible on the premise of achieving the expected purpose. The list of
excipients allowed to be used in enzyme preparations is shown in Appendix A.
4.1.6 The immobilized carrier used in the immobilized enzyme preparation shall
comply with the National food safety standard - Food contact materials and products
- Standards for uses of additives (GB 9685), National food safety standard - Standards
for uses of food additives (GB 2760) or other provisions on the list of raw materials
allowed to be used in food.
4.2 Physical and chemical indicators
Product enzyme activity shall meet the claims.
4.3 Pollutant limits
The pollutant limits shall comply with the provisions of Table 1.
4.4 Microbial limits
Microbial limits shall comply with the requirements in Table 2.Among them, producing
strains shall not be detected in enzyme preparations produced by microorganisms
obtained through genetic recombination technology.
4.5 Antibacterial activity
The antibacterial activity shall not be detected in enzyme preparations derived from
microorganisms, and the determination of the antibacterial activity is carried out in
accordance with the National food safety standard - Food microbiological examination
- Determination of antibacterial activity of enzyme preparations derived from microbial
origin (GB 4789.43).
Appendix A
List of excipients for enzyme preparations
The list of excipients for enzyme preparations is shown in Table A.1.
Table A.1 -- List of excipients for enzyme preparations
Appendix B
Reference method for determination of enzyme activity of some
enzyme preparations
B.1 Reference method for determination of enzyme activity of some enzyme
preparations
The reference methods for measuring the enzyme activity of some enzyme preparations
are shown in Table B.1.
B.3 Determination of pectinase activity
B.3.1 Pectinase
An enzyme that can hydrolyze pectin and generate products containing reducing groups.
B.3.2 Pectinase activity
Under the reaction conditions of 50 °C and pH 3.5, 1 g of solid enzyme powder (or 1
mL of liquid enzyme) decomposes pectin in 1 hour to produce 1 mg of galacturonic
acid, which is one enzyme activity unit, expressed in U/g or U/mL.
B.3.3 Principles
Pectinase can hydrolyze pectin, and the reducing uronic group of the generated
galacturonic acid can be quantitatively measured by the hypoiodous acid method to
express the activity of pectinase.
This method is mainly used to detect the activity of polygalacturonase and pectin lyase
in pectinase products. It is not suitable for pectin (methyl) esterase in pectinase products.
B.3.4 Reagents and materials
B.4 Determination of glucoamylase (amyloglucosidase) activity
B.4.1 Glucoamylase (amyloglucosidase)
Amyloglucosidase that uses starch as a substrate to hydrolyze α-1,4, α-1,6, α-1,3
glucosidic bonds from the non-reducing end of starch under certain conditions to
produce glucose.
B.4.2 Glucoamylase (amyloglucosidase) activity
1.0 mL of enzyme solution or 1.0 g of enzyme powder hydrolyzes soluble starch to
produce 1 mg of glucose in 1 hour at 40 °C and pH 4.6, which is 1 enzyme activity unit,
expressed in U/g (or U/mL).
B.4.3 Reagents and materials
B.4.3.1 0.05 mol/L acetic acid-sodium acetate buffer solution (pH 4.6). Weigh 6.7 g of
sodium acetate (CH3COONa • 3H2O), absorb 2.6 mL of glacial acetic acid, dissolve
with water and dilute to 1000 mL. The pH of the above buffer solution shall be
calibrated by using a pH meter.
B.4.4 Instruments and equipment
B.4.5 Determination procedure
B.4.5.1 Preparation of enzyme solution to be tested
B.4.5.2 Determination
Take two 50 mL colorimetric tubes, add 25 mL of soluble starch solution and 5 mL of
acetic acid-sodium acetate buffer solution respectively, and shake well. Preheat in a
constant-temperature water bath of 40 ℃±0.5 ℃ for 5 min~10 min. Add 2.0 mL of
enzyme solution to be tested into the first tube, time immediately, and shake well. After
accurately reacting at this temperature for 30 minutes,
B.4.6 Result calculation
The enzyme activity X4 of glucoamylase preparation, in U/g (or U/mL), is calculated
according to formula (B.4).
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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