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GB 1886.170-2016 PDF English


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GB 1886.170-2016: PDF in English

GB 1886.170-2016 NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard - Food Additives - 5'-guanylate disodium 食品添加剂 5'-鸟苷酸二钠 ISSUED ON: AUGUST 31, 2016 IMPLEMENTED ON: JANUARY 01, 2017 Issued by: National Health and Family Planning Commission of the PRC Table of Contents 1 Scope ... 3  2 Chemical name, structural formula, molecular formula, and relative molecular mass ... 3  3 Technical requirements ... 3  Appendix A Inspection methods ... 5  National Food Safety Standard - Food Additives - 5'-guanylate disodium 1 Scope This Standard applies to the food additive 5'-guanylate disodium prepared by fermentation or enzymatic hydrolysis using starch and saccharide as raw materials. 2 Chemical name, structural formula, molecular formula, and relative molecular mass 2.1 Chemical name 5'-guanylate disodium 2.2 Structural formula 2.3 Molecular formula C10H12N5Na2O8P • nH2O 2.4 Relative molecular mass 407.18 (according to 2011 international relative atomic mass, on anhydrous basis) 3 Technical requirements 3.1 Sensory requirements Spectrophotometer. A.3.2 Analytical procedures WEIGH 1 g of sample (accurate to 0.0001 g); dissolve in 20 mL of water; shake well. PLACE the above sample solution into a 1 cm cuvette; USE water as a blank control; USE a spectrophotometer to, at 430 nm, determine the light transmittance of the sample solution; RECORD the reading. The test result is based on the arithmetic mean of parallel determination results. The absolute difference between the two independent determination results, obtained under repeated conditions, shall not exceed 0.5% of the arithmetic mean. A.4 Determination of pH (5% aqueous solution) A.4.1 Instruments and equipment Acidimeter. A.4.2 Analytical procedures WEIGH 1 g of sample (accurate to 0.0001 g); dissolve in 20 mL of water; USE an acidimeter to determine the pH of the above sample solution. The test result is based on the arithmetic mean of parallel determination results. The absolute difference between the two independent determination results, obtained under repeated conditions, shall not exceed 0.2. A.5 Determination of other nucleotides A.5.1 Reagents and materials A.5.1.1 Developing solution: tert-butanol+saturated ammonium sulfate+0.025 mol/L aqueous ammonia=3+160+40. A.5.1.2 Chromatographic filter paper. A.5.2 Instruments and equipment A.5.2.1 UV analyzer. A.5.2.2 Microinjector. A.5.3 Analytical procedures WEIGH 1.0 g of sample; ADD water to dissolve and dilute to 100 mL. USE a chromatographic filter paper for application of 10.0 μL of sample; SPREAD ......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.