GB/T 28642-2012 English PDF

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GB/T 28642-2012: Rapid determination of Salmonella spp.in animal feeding stuffs -- PCR method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 28642-2012	229	Add to Cart	3 days	Rapid determination of Salmonella spp.in animal feeding stuffs -- PCR method	Valid

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Basic data

Standard ID: GB/T 28642-2012 (GB/T28642-2012)
Description (Translated English): Rapid determination of Salmonella spp.in animal feeding stuffs -- PCR method
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 10,164
Quoted Standard: GB/T 6682; GB/T 13091; GB/T 14699.1; GB 19489; SN/T 1193; SN/T 1870
Regulation (derived from): National Standards Bulletin 2012 No. 17
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard provides rapid detection of Salmonella in feed by PCR method. This standard applies to qualitative screening of Salmonella in feed.

GB/T 28642-2012: Rapid determination of Salmonella spp.in animal feeding stuffs -- PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS65.120 B46 National Standards of People's Republic of China Rapid Detection of Salmonella in feed Polymerase chain reaction (PCR) method PCRmethod Issued on. 2012-11-01 2012-07-31 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard rule according to GB/T 1.1-2009 given draft. This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized. This standard was drafted. the Ministry of Agriculture and livestock feed quality and safety Supervision and Testing Center (Shenyang). The main drafters. Zhang Xiuqin, TRANSACTIONS, Du Bolin, Lixin Nan, Yang Xiguo, Ma Chi, Chen months. Rapid Detection of Salmonella in feed Polymerase chain reaction (PCR) method

1 Scope

This standard specifies the PCR method for rapid detection of Salmonella in feed for. This standard applies to feed Salmonella qualitative screening.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated edition applies to this article Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 13091 salmonella in feed detection method GB/T 14699.1 feed samples GB 19489 Laboratory Biosafety General Requirements SN/T1193 genetic testing laboratory technical requirements SN/T1870 pathogens in food real-time PCR detection methods Principle 3 Use boiling water bath so that bacterial cells rupture, releasing genomic DNA, centrifugation cell wall visible precipitate in the supernatant as a template Amplification and agarose gel electrophoresis to detect PCR amplification products.

4 Reagents and materials

Unless otherwise specified, reagents were analytical grade or biochemical reagents, test water meet the requirements of GB/T 6682's. 4.1 Salmonella detection primer (pair) sequence. SalF. 5'-TCGCACCGTCAAAGGAACCGTAAAGC-3 ' SalR. 5'-GCATTATCGATCAGTACCAGCCGTCT-3 ' 4.2 PremixTaq buffer (2 ×). contains TaqDNA polymerase 1.25U/25μL, 4 mmolMg2, dNTP Each 0.4mmol. 4.3 agarose. electrophoresis grade. 4.4 Marker2000. 4.5 buffered peptone water (BPW). See A.1. 4.6 Soy peptone broth (RVS). See A.2. 4.7 selenite cystine enrichment broth (SC). See A.3. 4.8 10 times in the sample buffer (10 × loadingbuffer). 0.05% bromophenol blue, 50% glycerol solution, 1% SDS. 4.9 Quality control strains. Salmonella positive standard strains. 4.10 50 × TAE buffer. see A.4. ......

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