GB/T 17818-2025 English PDFUS$439.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 17818-2025: Determination of vitamin D3 in feeds - High-performance liquid chromatography Status: Valid GB/T 17818: Historical versions
Basic dataStandard ID: GB/T 17818-2025 (GB/T17818-2025)Description (Translated English): Determination of vitamin D3 in feeds - High-performance liquid chromatography Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B46 Classification of International Standard: 65.120 Word Count Estimation: 22,295 Date of Issue: 2025-01-24 Date of Implementation: 2025-08-01 Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration GB/T 17818-2025: Determination of vitamin D3 in feeds - High-performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.ICS 65.120 CCSB46 National Standard of the People's Republic of China Replace GB/T 17818-2010 Determination of Vitamin D3 in Feed High performance liquid chromatography Released on 2025-01-24 2025-08-01 Implementation State Administration for Market Regulation The National Standardization Administration issued ForewordThis document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. This document replaces GB/T 17818-2010 "Determination of vitamin D3 in feed by high performance liquid chromatography". Compared with.2010, in addition to structural adjustments and editorial changes, the main technical changes are as follows. a) The scope of application has been changed, the concentrate supplement has been added, and the quantitative limit of the “first method saponification extraction method” has been changed (see Chapter 1,.2010 Chapter 1 of the.2001 edition); b) The quantification limit of the “second method direct extraction method” has been changed (see Chapter 1, Chapter 1 of the.2010 edition); c) Added the “first method saponification extraction method” vitamin D3 standard series solution (see 4.2.21); d) The sample preparation method for compound feed, concentrate supplement and concentrated feed in the “First Method of Saponification Extraction” has been changed to Pass through a 1mm sieve (see 4.4, 3.5 of the.2010 edition); e) Changed the extractant of "First Method Saponification Extraction Method" to "petroleum ether" (boiling range 30℃~60℃) (see 4.5.1.2.1,.2010 Edition) 3.6.1.2); f) Added off-line solid phase extraction method (see 4.5.1.2.2) and online solid phase extraction method (see 4.5.1.2.3); g) Added liquid chromatography reference conditions (see 4.5.2.2, 4.5.2.3); h) Added qualitative detection methods and added multi-point calibration for quantitative detection (see 4.5.2.5, 4.5.2.6); i) Deleted the HPLC purification column purification (see 3.6.1.4 of the.2010 edition); j) Deleted the HPLC purification conditions (see 3.6.2.1 of the.2010 edition); k) Normal phase chromatography was deleted (see 3.6.2.2.1 of the.2010 edition); l) Added precision requirements for vitamin D3 content in the range of 100 IU/kg to 1000 IU/kg (see 4.7,.2010 edition) 3.6.2.5); m) The “Second Method Direct Extraction Method” has been changed. The water bath temperature should not exceed 65°C (see 5.5.1, 4.6.1 of the.2010 edition); n) Added qualitative detection method (see 5.5.2.3). Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences, Shandong Provincial Livestock Product Quality and Safety Center Heart, DSM Vitamins (Shanghai) Co., Ltd., Sichuan Well Testing Technology Co., Ltd., China Animal Husbandry Industry Co., Ltd., Guangzhou Aibao Agribiotech Ltd. The main drafters of this document are. Zhao Xiaoyang, Yu Zhegao, Song Rong, Zhang Fengping, Zhu Gaoqun, Liu Zhiying, Wang Zhongyan, Guo Hongshuang, Ma Xiaozhong, Zhang Wei, Xie Li, Song Yan, Zhang Hui, Li Libei, Chen Xuehai, Chen Xue, Cui Jie, Feng Xiuyan, Cao Lin. The previous versions of this document and the documents it replaces are as follows. ---First published in.1999 as GB/T 17818-1999, first revised in.2010; ---This is the second revision. Determination of Vitamin D3 in Feed High performance liquid chromatography1 ScopeThis document describes a method for the determination of vitamin D3 in feeds by HPLC. The "first method of saponification extraction" in this document is applicable to vitamins in compound feeds, concentrate supplements, concentrated feeds, and compound premixed feeds. Determination of vitamin D3.“The second method direct extraction method” is suitable for the determination of vitamin D3 in vitamin premix feed. The first limit of measurement in this document is 100 IU/kg, and the second limit of measurement is 2.00×105 IU/kg.2 Normative referencesThe contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples3 Terms and definitionsThere are no terms or definitions that require definition in this document. 4.Method 1.Saponification Extraction Note. The glass surface of the separatory funnel piston is not oiled, the handling process is carried out under light protection; the extraction process is carried out in a fume hood. 4.1 Principle The sample was saponified with potassium hydroxide ethanol solution, purified and concentrated by liquid-liquid extraction or solid phase extraction, and separated by two-dimensional chromatography system. The compound premixed feed can also be directly separated by reverse phase chromatography column, detected by UV detector and quantified by external standard method. 4.2 Reagents or materials Unless otherwise specified, only analytical grade reagents were used. 4.2.1 Water. GB/T 6682, Grade 1. 4.2.2 Anhydrous ethanol. chromatographic grade. 4.2.3 Anhydrous ethanol. 4.2.4 Petroleum ether (boiling range 30℃~60℃). 4.2.5 Methanol. chromatographic grade. 4.2.6 Acetonitrile. chromatographic grade. 4.2.7 Formic acid. chromatographically pure. 4.2.8 Isopropanol. chromatographic grade. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 17818-2025_English be delivered?Answer: Upon your order, we will start to translate GB/T 17818-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 17818-2025_English with my colleagues?Answer: Yes. 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