GB/T 28230-2011 English PDFUS$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 28230-2011: Enumeration of coliforms in ballast water of entry-exit ships by membrane filtration Status: Valid
Basic dataStandard ID: GB/T 28230-2011 (GB/T28230-2011)Description (Translated English): Enumeration of coliforms in ballast water of entry-exit ships by membrane filtration Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: C51 Classification of International Standard: 07.100.20 Word Count Estimation: 7,742 Date of Issue: 2011-12-30 Date of Implementation: 2012-05-01 Adopted Standard: EPA Method 1604-2002, MOD Regulation (derived from): Announcement of Newly Approved National Standards No. 23 of 2011 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the use of membrane filtration method for the determination of entry and exit of ships' ballast water coliform procedures and methods. This standard applies to the entry and exit of ships' ballast water and coliform counts. GB/T 28230-2011: Enumeration of coliforms in ballast water of entry-exit ships by membrane filtration---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Enumeration of coliforms in ballast water of entry-exit ships by membrane filtration ICS 07.100.20 C51 National Standards of People's Republic of China Membrane filtration method entry and exit of ships Ballast water coliform Issued on. 2011-12-30 2012-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. This revised standards used by the US Environmental Protection Agency (EPA) "Method 1604. Membrane filtration method Combined Determination of total coliforms and E. coli Bacteria "(September 2002) [Method1604. TotalcoliformsandEscherichiacoliinwaterbymembranefiltration usingasimultaneousdetectiontechnique (MImedium), september2002]. The main difference between this and the standard EPA methods are. --- Remove the E. coli count of content; --- Incubator temperature from 35 ℃ ± 0.5 ℃ revised to 36 ℃ ± 1 ℃; --- Increase the water sample dilution steps. This standard was proposed by the State Administration of Quality Supervision, Inspection and Quarantine. The standard by the China Academy of Inspection and Quarantine. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Qinhuangdao Exit Inspection and Quarantine, the Chinese people Exit Inspection and Quarantine of the Republic of Guangzhou. The main drafters of this standard. Wang Lin, co-Shun, Zhang Yue, Lijun Cheng, there are Li, Nie Weizhong, Liao Ruyan, Chen Chuntian, Chang Lin, Chi Ying, Pangqiu Yan, Yang Chenguang, Han Hui. Membrane filtration method entry and exit of ships Ballast water coliform1 ScopeThis standard specifies the method for determining entry and exit procedures and the ship's ballast water and coliform bacteria (coliforms) using membrane filtration method. This standard applies to the entry and exit of ships' ballast water and coliform counts.2 Equipment and Materials2.1 incubator. 36 ℃ ± 1 ℃. 2.2 Refrigerator. 2 ℃ ~ 8 ℃. 2.3 100mL graduated cylinder. sterilization. 2.4 membrane filtering device 1). see 4.1.2. 1) You can use membrane filtration device equivalent. 2.5 Sterile cellulose ester membrane, white, with a grille, 47mm diameter, pore size 0.45μm. 2.6 Sterile pipettes. 10mL, accurate to 0.1mL. 2.7 sterile saline. 2.8 conical flask. 150mL. 2.9 UV lamp. wavelength 366nm. 2.10 sterile Petri dish. a diameter of 50mm. 2.11 sterile filter. pore size of 0.22μm. 2.12 MI medium. See Appendix A.3 testColiform detection program shown in Figure 1.4 steps4.1 Preparation 4.1.1 Preparation of MI agar medium, preservation medium should be 4 ℃ temperature returned to room temperature before use. 4.1.2 Assembly filtration device. using sterile forceps sterile membrane filter is placed on a porous plate, facing the grill, filter bowl and filter assembly base Block connection buffer bottle, vacuum pump (not available syringe pumps in place). Figure 2 illustrates the filter device. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 28230-2011_English be delivered?Answer: Upon your order, we will start to translate GB/T 28230-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of GB/T 28230-2011_English with my colleagues?Answer: Yes. 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