GB/T 21676-2008 English PDF

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GB/T 21676-2008: Milk and dairy products -- Determination of fatty acid content by gas -- Liquid chromatography
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 21676-2008	279	Add to Cart	3 days	Milk and dairy products -- Determination of fatty acid content by gas -- Liquid chromatography	Obsolete

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Basic data

Standard ID: GB/T 21676-2008 (GB/T21676-2008)
Description (Translated English): Milk and dairy products -- Determination of fatty acid content by gas -- Liquid chromatography
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C53
Classification of International Standard: 67.100.01
Word Count Estimation: 7,717
Date of Issue: 2008-04-09
Date of Implementation: 2008-06-01
Quoted Standard: GB/T 5413.28-1997; GB/T 6682
Adopted Standard: ISO 15885-2002, NEQ
Regulation (derived from): National Standard Approval Announcement 2008 No.6 (Total No.119); National Standard Approval Announcement 2010 No.5 (Total No.160)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the gas chromatographic determination of milk and dairy products of fatty acids. This standard applies to the determination of milk and dairy products of fatty acids.

GB/T 21676-2008: Milk and dairy products -- Determination of fatty acid content by gas -- Liquid chromatography

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Milk and dairy products. Determination of fatty acid content by gas. Liquid chromatography ICS 67.100.01 C53 National Standards of People's Republic of China Determination of milk and milk products of fatty acids Gas chromatography (ISO 15885.2002/IDF184.2002, Milkfat-Determinationofthefattyacid compositionbygas-liquidchromatography, NEQ) Posted 2008-04-09 2008-06-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard is not equivalent to ISO 15885.2002/IDF184.2002 "Gas Chromatography fatty acid composition of milk fat." (In English). Appendix A of this standard is an informative annex. The standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee of animal husbandry. This standard was drafted. the Ministry of Agriculture Food Quality Supervision and Testing Center (Shanghai). The main drafters of this standard. Meng Jin, Zheng tree crown, Han Yi Yi, Wu Rong, Chen Meilian, Zhang Hui. Determination of milk and milk products of fatty acids Gas chromatography

1 Scope

This standard specifies the gas chromatographic method for milk and dairy products of fatty acids. This standard applies to the determination of milk and dairy products of fatty acids.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 5413.27-1997 Milk powder and infant formula DHA, EPA's determination GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-1992, neq ISO 3696. 1987) Principle 3 Milk fat production of free fatty acids after saponification treatment in boron trifluoride catalyzed by fatty acid methyl esters by gas chromatography column after points Off to a flame ionization detector and external standard quantification.

4 Reagents

Unless otherwise specified, use only analytical reagent. 4.1 Water. GB/T 6682 provided a water. 4.2 anhydrous sodium sulfate (Na2SO4). 4.3 heptane [CH3 (CH2) 5CH3]. chromatography. 4.4 methanol solution of boron trifluoride. 10% mass fraction. Note. For each batch of new reagent or solvent, particularly methanol solution of boron trifluoride, should be prepared by pure oleic acid methyl ester and chromatographic analysis, if 20 to 22 carbon Interfering peaks in the fatty area is not available. 4.5 saturated sodium chloride solution. dissolve 360g of sodium chloride (NaCl) in 1L of water, stirring to dissolve, clarify the reserve. 4.6 sulfuric acid solution (20% volume fraction). 20mL of diluted sulfuric acid (H2SO4) to 100mL, and mix. 4.7 methanol solution of potassium hydroxide [Ba (KOH) = 2mol/L]. Weigh 11.2g of potassium hydroxide (KOH), dried over anhydrous methanol (CH3OH) was dissolved Solution and diluted to volume to 100mL, and mix. 4.8 FAME standard stock solution. Each mL of fatty acid methyl esters containing 10.0mg. Weigh accurately FAME standard substance 0.50g in 50mL brown volumetric flask, with heptane (4.3) was dissolved constant volume, this solution per ml L fatty acid methyl esters containing 10.0mg. Shake well and stored at -18 ℃ refrigerator, valid 360d. 4.9 mixed fatty acid methyl ester standards working solution. Each ml of each fatty acid methyl esters 0.02mg. Accurate pipetting 1.0mL each fatty acid methyl ester standard solution (4.8) in 50mL brown volumetric flask, with heptane (4.3) diluted to volume; re-registration Indeed Pipette 1.0mL to 10mL brown volumetric flask, with heptane (4.3) diluted to volume, the solution per ml of each fatty acid methyl ester 0.02mg. Shake well and stored at -18 ℃ refrigerator, valid 90d. 4.10 remaining reagents with GB/T 5413.27-1997 reagents.

5 Equipment

Common laboratory equipment and the following. ......

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