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GB/T 17480-2008 English PDF

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Q: Should I purchase the latest version GB/T 17480-2008?

Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 17480-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.

US$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 17480-2008: Determination of aflatoxin B1 in animal feeding stuffs -- Enzyme-linked immunosorbent assay
Status: Valid

GB/T 17480: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 17480-2008	189	Add to Cart	3 days	Determination of aflatoxin B1 in animal feeding stuffs -- Enzyme-linked immunosorbent assay	Valid
GB/T 17480-1998	279	Add to Cart	3 days	Determination of aflatoxin B1 in feeds--Enzyme-linked immunosorbent assay	Obsolete

Similar standards

GB/T 17819 GB/T 17810 GB/T 17811 GB/T 17818 GB/T 17812 GB/T 17481

Basic data

Standard ID: GB/T 17480-2008 (GB/T17480-2008)
Description (Translated English): Determination of aflatoxin B1 in animal feeding stuffs -- Enzyme-linked immunosorbent assay
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B46
Classification of International Standard: 65.120
Word Count Estimation: 8,836
Date of Issue: 2008-11-21
Date of Implementation: 2009-02-01
Older Standard (superseded by this standard): GB/T 17480-1998
Quoted Standard: GB/T 20195
Regulation (derived from): National Standard Approval Announcement 2008 No.19 (Total No.132)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary: This standard specifies the ELISA method for aflatoxin B1 in feed. This standard applies to all kinds of feed ingredients, feed and determination of aflatoxin B1 in feed concentrates. This standard detection limit of 0. 1��g/kg.

GB/T 17480-2008: Determination of aflatoxin B1 in animal feeding stuffs -- Enzyme-linked immunosorbent assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of aflatoxin B1 in animal feeding stuffs. Enzyme-linked immunosorbent assay ICS 65.120 B46 National Standards of People's Republic of China Replacing GB/T 17480-1998 Determination of aflatoxin B1 aflatoxin in feed Enzyme-linked immunosorbent assay Posted 2008-11-21 2009-02-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard replaces GB/T 17480-1998 "feed aflatoxin B1 measured by ELISA." This standard and GB/T 17480-1998 compared to major changes are as follows. --- Range increase the "standard detection limit of 0.1μg/kg"; --- Normative documents, "GB/T 20195 preparation of animal feed samples" used instead of "GB/T 8381-1987 feed Determination "of aflatoxin B1; --- Kit composition to increase the "Note. Product composition and operation of the different test case between manufacturers will be slightly different, should be strictly in accordance with Specification requirements and standards "; --- Instruments, equipment provision, remove the refrigerator terms; increased electric oscillator, grinding stoppered flask; --- The "sample" clause to "sample preparation", while the "Press GB/T 8381-1987 5.1 requirements collection, sample handling, system To analyze the sample using "to" press GB/T 20195 requirements for specimen preparation "; --- Limited Terms determination, the determination of the terms of grading operations into a single provision, and delete tables are measured in narrative process; --- Quantitative determination of the terms of increase "result holds two significant figures"; --- The original "other" clause deleted, "where contact with AFB1 container needs dipped 1% sodium hypochlorite (NaClO2) solution, 12h after cleaning spare. In order to analyze the safety of personnel, to bring medical latex gloves "to" Warning "Terms of operation; --- Increase the informative Appendix A; --- The sample solution is diluted and the dilution table with the results of the calculation as an information table in Appendix B. The Standard Appendix A and Appendix B are informative. This standard by the National Standardization Technical Committee Feed Industry and focal points. This standard was drafted. Jiangsu Provincial Institute of Microbial limited liability company. The main drafters of this standard. Li Lidong, Mi Xiaoli, Yuan Jianxing, Du Shu Lin. This standard was first published in 1998, this is the first revision. Determination of aflatoxin B1 aflatoxin in feed Enzyme-linked immunosorbent assay

1 Scope

This standard specifies the feed of aflatoxin B1 enzyme linked immunosorbent assay (ELISA) method. This standard applies to all kinds of feed materials, compound feed and determination of aflatoxin B1 in feed concentrate. The standard detection limit of 0.1μg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 20195 preparation of animal feed samples (GB/T 20195-2006, ISO 6498.1998, IDT) Principle 3 Sample aflatoxin B1, aflatoxin B1 ELISA antigen coated on microplate aflatoxin B1 specific anti Body is immune competitive reaction, after addition of the enzyme substrate color, the color of the sample is inversely proportional to the aflatoxin content of aflatoxin B1. Were visually or Instrumental method by standard solution of aflatoxin B1 in the sample comparison determination or calculation aflatoxin content of aflatoxin B1.

4 Reagents and materials

Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity. 4.1 Aflatoxin B1 ELISA test kit reagents Note. Products of different test kit manufacturer between the composition and operation will be slightly different, should be strictly in accordance with specification requirements and standards. 4.1.1 coated with anti-aflatoxin B1 antibody polystyrene microtiter plate. 4.1.2 Sample dilution. Methanol - Distilled water (93 + 7). 4.1.3 aflatoxin B1 standard solution. 1.00μg/L, 50.00μg/L. Security analysts, to bring medical latex gloves when operating. 4.1.4 ELISA antigen Aflatoxin B1. aflatoxin B1- horseradish peroxidase crosslinked. 4.1.5 0.01mol/phosphate buffer LpH7.5 preparation. Weigh 3.01g of disodium hydrogen phosphate (Na2HPO4 · 12H2O), 0.25g sodium dihydrogen phosphate (NaH2PO4 · 2H2O), 8.76g of sodium chloride (NaCl), dissolved in water to 1L. 4.1.6 ELISA Aflatoxin B1 antigen dilution solution. Weigh 0.1g of bovine serum albumin (BSA) dissolved in phosphate buffered 100mLpH7.5 Red solution (4.1.5). 4.1.7 0.1mol/LpH7.5 phosphate buffer solution. Weigh 30.1g of disodium hydrogen phosphate (Na2HPO4 · 12H2O), 2.5g dicalcium phosphate Sodium hydroxide (NaH2PO4 · 2H2O), 87.6g of sodium chloride (NaCl), dissolved in water to 1L. 4.1.8 wash liquor. lessons 0.5mL Tween-20 in 1000mL0.1mol/LpH7.5 phosphate buffer (4.1.7). 4.1.9 pH5.0 sodium acetate - citric acid buffer solution. Weigh 15.09g sodium acetate (CH3COONa · 3H2O), 1.56g of citric acid (C6H8O7 · H2O), dissolved in water to 1L. 4.1.10 substrate solution a. Weigh tetramethylbenzidine (TMB) 0.2g sodium acetate dissolved 1LpH5.0 - citrate buffer. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 17480-2008_English be delivered?

Answer: Upon your order, we will start to translate GB/T 17480-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 17480-2008_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 17480-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.

Question 5: Should I purchase the latest version GB/T 17480-2008?

Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 17480-2008 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.