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SN/T 4283.5-2015: Test method of microplate gene chip at frontier port. Part 5: Mycoplasma pneumonia, chlamydia pneumonia and legionella pneumophila
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Test method of microplate gene chip at frontier port. Part 5: Mycoplasma pneumonia, chlamydia pneumonia and legionella pneumophila
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SN/T 4283.5-2015
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Basic data | Standard ID | SN/T 4283.5-2015 (SN/T4283.5-2015) | | Description (Translated English) | Test method of microplate gene chip at frontier port. Part 5: Mycoplasma pneumonia, chlamydia pneumonia and legionella pneumophila | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 11.020 | | Word Count Estimation | 8,872 | | Date of Issue | 2015-05-26 | | Date of Implementation | 2016-01-01 | | Quoted Standard | GB 19489; SN/T 2351; SN/T 2752.4-2011; SN/T 2770; SN/T 4283.1; WS 233 | | Regulation (derived from) | State Quality-Inspection-accreditation [2015] No.224 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the border crossings three kinds of atypical pneumonia pathogens (Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila) microplate microarray detection method. This standard applies to border crossings in the laboratory using a microplate gene chip technology for three kinds of atypical pneumonia pathogens (Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila) detection. | SN/T 4283.5-2015: Test method of microplate gene chip at frontier port. Part 5: Mycoplasma pneumonia, chlamydia pneumonia and legionella pneumophila
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(Microarray microplate border crossings - Part 5. Mycoplasma pneumoniae, Chlamydia pneumoniae and Legionella pneumophila)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Border crossings microplate microarray detection method
Part 5. Mycoplasma pneumoniae, Chlamydia pneumoniae and
Legionella pneumophila
Testmethodofmicroplategenechipatfrontierport-Part 5. Mycoplasma
pneumonia, chlamydiapneumoniaandlegionelapneumophila
Issued on. 2015-05-26
2016-01-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 4283 "border crossings microplate microarray detection method" standard for the series, is divided into six parts.
--- Part 1. General technical specification;
--- Part 2. Mycobacterium tuberculosis and resistance mutations katG and rpoB genes;
--- Part 3. 7 respiratory viruses;
--- Part 4. intestinal virus and enterovirus 71, coxsackievirus A16;
--- Part 5. Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila;
--- Part 6. 12 kinds of food-borne pathogens.
This section SN/T Section 54283 of.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, the Chinese people
Republic of Sichuan Entry-Exit Inspection and Quarantine Xinjiang People's Republic of China, Standard Instrument Co., Ltd. Zhuhai fine.
The main drafters of this section. Jianan, Fan Xuejun, Xu Yuan, Gao Long, Liu Ling, Zhao pure in history Lei, LIU Chun-xiao, Zhao Fang, Xu Yunqing,
Eric Lee.
Border crossings microplate microarray detection method
Part 5. Mycoplasma pneumoniae, Chlamydia pneumoniae and
Legionella pneumophila
1 Scope
SN/T 4283 provisions of this part of the frontier port three kinds of atypical pneumonia pathogens (Mycoplasma pneumoniae, Chlamydia pneumoniae and L. pneumophila army
Mission bacteria) microplate microarray detection method.
This section applies to border crossings in the laboratory using a microplate mycoplasma gene chip technology for three kinds of atypical pneumonia pathogens (pneumonia,
Chlamydia pneumoniae and Legionella pneumophila) detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
SN/T 2351 port of entry inspection of Legionella
SN/T 2752.4-2011 specification of self-protection of the health and quarantine personnel - Part 4. Laboratory personnel
SN/T 2770 frontier port Legionella PCR assay method
SN/T 4283.1 frontier port microplate chip detection methods - Part 1. General technical specification
WS233 Microbiological and Biomedical Laboratories Biosafety Common Criteria
3 Terms and Definitions
SN/T 4283.1 and define the following terms and definitions apply to this document.
3.1
SARS atypicalpneumonia
With respect to typical pneumonia terms, and more from mycoplasma, chlamydia, legionella, rickettsia, viruses and other microorganisms unknown
Pathogen causes, symptoms and pulmonary symptoms, blood test results are not typical pneumonia is clear that some viral pneumonia antibiotics ineffective.
3.2
Mycoplasma pneumoniae mycoplasmapneumonia
Cause atypical pneumonia common pathogens, size of about 0.2μm ~ 0.3μm, appear to be highly polymorphic nature, no cell wall,
The cytoplasm contains a circular double-stranded DNA separated from the cytoplasm, it is the smallest genome of a prokaryotic cell.
3.3
Chlamydia pneumoniae chlamydiapneumonia
The main cause of atypical pneumonia, the original body shape diversity, rounded nucleus, located in the center of the cell, there is wide between the original nucleus and cell membrane
Biomass district. Chlamydia pneumoniae can not be cultured in vitro, only parasites in the cells, chicken embryo is insensitive, it is generally used in cell culture passage.
Since Chlamydia pneumoniae infection is no typical clinical manifestations, diagnosis rely mainly on laboratory testing.
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