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Test method of microplate gene chip at frontier port - Part 2: Mycobacterium tuberculosis and drug resistant mutations of katG and rpoB genes
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Basic data Standard ID | SN/T 4283.2-2015 (SN/T4283.2-2015) | Description (Translated English) | Test method of microplate gene chip at frontier port - Part 2: Mycobacterium tuberculosis and drug resistant mutations of katG and rpoB genes | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | C62 | Word Count Estimation | 10,191 | Date of Issue | 2015-05-26 | Date of Implementation | 2016-01-01 | Quoted Standard | GB 19489; SN/T 1262; SN/T 2752.4-2011; SN/T 3312; SN/T 4283.1; WS 233 | Regulation (derived from) | State Quality-Inspection-accreditation [2015] No.224 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the frontier port and microplate Mycobacterium tuberculosis DNA microarray detection of rpoB and katG gene mutation. This standard applies to border crossings in the laboratory using a microplate gene chip technology, and Mycobacterium tuberculosis katG and rpoB genes two resistance mutations were detected. |
SN/T 4283.2-2015: Test method of microplate gene chip at frontier port - Part 2: Mycobacterium tuberculosis and drug resistant mutations of katG and rpoB genes ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of microplate gene chip at frontier port - Part 2. Mycobacterium tuberculosis and drug resistant mutations of katG and rpoB genes
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Micro-plate microchip detection method at border port
Part 2. Mycobacterium tuberculosis and katG
And rpoB resistance variant gene
Part 2. Mycobacteriumtuberculosisanddrugresistant
Released on.2015-05-26
2016-01-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4283 "Methods for Detection of Microplates in National Ports" is a series of standards, which are divided into six parts.
--- Part 1. General technical procedures;
--- Part 2. Mycobacterium tuberculosis and katG and rpoB resistance variant genes;
--- Part 3. 7 respiratory viruses;
--- Part 4. Enterovirus and enterovirus 71, Coxsackievirus A16;
--- Part 5. Mycoplasma pneumoniae, Chlamydia pneumoniae and Legionella pneumophila;
--- Part 6. 12 foodborne pathogens.
This part is the second part of SN/T 4283.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Shenzhen South
Mountain Chronic Disease Prevention and Control Institute, Zhuhai Fine Standard Instrument Co., Ltd.
The main drafters of this section. Liu Chunxiao, Yang Yanqiu, Zhang Tao, Zhang Shuping, Liu Jun, Gu Dayong, Shi Lei, Zhao Chunzhong, He Jianan, Xu Yunqing,
Li Yongjin.
Micro-plate microchip detection method at border port
Part 2. Mycobacterium tuberculosis and katG
And rpoB resistance variant gene
1 Scope
This section of SN/T 4283 specifies microplate genes for M. tuberculosis and katG and rpoB resistance variant genes at border ports.
Chip detection method.
This section applies to the use of microplate microarray technology in the border port laboratory for M. tuberculosis and katG and rpoB
Drug resistance variant genes were tested.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
SN/T 1262 Border Inspection Procedures for Tuberculosis
SN/T 2752.4-2011 Self-protection regulations for health and quarantine personnel - Part 4. Laboratory personnel
SN/T 3312 Mycobacterium tuberculosis γ-interferon in vitro detection method
SN/T 4283.1 Micro-plate chip testing method for border ports - Part 1. General technical specification
WS233 Microbiological and Biomedical Laboratory General Guidelines for Biosafety
3 Terms and definitions
The following terms and definitions defined by SN/T 4283.1 and SN/T 1262, SN/T 3312 apply to this document.
3.1
Drug resistance variant gene
A gene that causes resistance due to insertion, deletion, substitution, etc. of a base. Resistance gene mutation is resistant to M. tuberculosis
The main mechanism of medicinal properties.
3.2
katG gene katGgene
A gene encoding catalase-peoxidase.
3.3
rpoB gene rpoBgene
Coding of the DNA-dependent RNA polymerase (DPRP) beta subunit of Mycobacterium tuberculosis
gene.
4 Abbreviations
The following abbreviations, as defined by SN/T 4283.1, apply to this document.
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