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SN/T 4283.1-2015 English PDF

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SN/T 4283.1-2015: Test method of microplate gene chip at frontier port. Part 1: General technical procedure
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Basic data

Standard ID SN/T 4283.1-2015 (SN/T4283.1-2015)
Description (Translated English) Test method of microplate gene chip at frontier port. Part 1: General technical procedure
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C62
Classification of International Standard 11.020
Word Count Estimation 11,147
Date of Issue 2015-05-26
Date of Implementation 2016-01-01
Quoted Standard GB 19489; SN/T 2752.4-2011; WS 233
Regulation (derived from) State Quality-Inspection-accreditation [2015] No.224
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the general technical specification microplate microarray detection methods, including preparation, microplate gene chip testing process, the results and the disposal of part of the report positive results. This standard applies to border crossings in the laboratory using a microplate gene chip technology for detection.

SN/T 4283.1-2015: Test method of microplate gene chip at frontier port. Part 1: General technical procedure


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Test method of microplate gene chip at frontier port.Part 1. General technical procedure People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Micro-plate microchip detection method at border port Part 1. General technical procedures Part 1. Generaltechnicalprocedure Released on.2015-05-26 2016-01-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued Entry and exit inspection and quarantine Industry Standard Micro-plate microchip detection method at border port Part 1. General technical procedures China Standard Press Publishing First edition in February.2016

Foreword

SN/T 4283 "Methods for Detection of Microplates in National Ports" is a series of standards, which are divided into six parts. --- Part 1. General technical procedures; --- Part 2. Mycobacterium tuberculosis and katG and rpoB resistance variant genes; --- Part 3. 7 respiratory viruses; --- Part 4. Enterovirus and enterovirus 71, Coxsackievirus A16; --- Part 5. Mycoplasma pneumoniae, Chlamydia pneumoniae and Legionella pneumophila; --- Part 6. 12 foodborne pathogens. This part is the first part of SN/T 4283. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Chinese people Xinjiang Entry-Exit Inspection and Quarantine Bureau, Sichuan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Gansu Exit and Entry Inspection of the People's Republic of China Inspection and Quarantine Bureau, Henan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Tsinghua University Shenzhen Graduate School, Shenzhen Nanshan District Chronic Disease Prevention and Control Institute, Zhuhai Fine Standard Instrument Co., Ltd., Shenzhen Tupu Technology Co., Ltd. The main drafters of this section. Gu Dayong, Xin Benqiang, Dong Ruiling, Liao Ling, Fan Xuejun, Gao Guolong, Liu Fang, Zhao Fang, Liu Chunxiao, Yang Yanqiu, Zhang Shuping, Shi Lei, Zhao Chunzhong, He Jianan, Liu Jun, Xu Yuan, Lin Qinfeng, Xu Yunqing, Zhang Qin, Huang Enzhen, Ma Wei, Zhang Tao, Li Yongjin, Ni Xiu. Micro-plate microchip detection method at border port Part 1. General technical procedures

1 Scope

This part of SN/T 4283 specifies general technical specifications for microplate microarray detection methods, including the preparation of microplate gene chips. Preparation, testing process, results report and disposal of positive results. This section applies to the detection of microplate genetic chip technology in the border port laboratory.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 19489 General requirements for laboratory biosafety SN/T 2752.4-2011 Self-protection regulations for health and quarantine personnel - Part 4. Laboratory personnel WS233 Microbiological and Biomedical Laboratory General Guidelines for Biosafety

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Microplate gene chip microplategenechip Different from the traditional slide-based or membrane-based planar gene chip, the gene probe is fixed in the micropores in a preset mode, and each hole is A chip. The microplate gene chip technology involved in this section has the following characteristics. (a) the carrier of the chip is a microporous plate made of polymer material. Similar to an 8×12 ELISA plate, each well is a chip. Can use up to 96 reaction wells on the whole plate, or The number of reaction wells required for disassembly and assembly needs to be traced and high-throughput. (b) The reaction surface of the chip does not need to be modified or modified by an organic thin film coating. The modification of the student's object can directly spot the gene probe to the surface of the hole, and the hydrogen bond is combined under the action of ultraviolet light, so that the coated probe is not easy to take off. It has the characteristics of simple and convenient chip preparation operation. (c) The chip detection adopts the detection method of chemical color reaction, and the detection principle is raw The materialized PCR product hybridizes with the chip and forms a color point on the surface of the chip via streptavidin-alkaline phosphatase-mediated color reaction. Accumulation, detection results can also be quantitatively analyzed by means of a chip scanner, which is fast and easy to visualize. 3.2 Specific probe specificprobe A fragment of an oligonucleotide molecule capable of hybridizing to a specific target nucleic acid in a sample for detection purposes. 3.3 Positive control probe positive controlprobe An oligonucleotide molecule fragment that hybridizes to a predetermined target nucleic acid but does not cross-hybridize with the detection target nucleic acid. Used to achieve quality control of all or part of the inspection process. This section deals with PCR positive control probes and hybrid positive control probes. 3.4 Negative control probe negativecontrolprobe That is, a blank control probe, which may be an artificially designed synthetic oligonucleotide that has no paired hybridization or is unrelated to the assay.

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