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SN/T 3929-2014 English PDF

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SN/T 3929-2014: Determination of L-hydroxyproline in foodstuffs for export. LC-MS/MS method
Status: Valid
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SN/T 3929-2014English519 Add to Cart 4 days [Need to translate] Determination of L-hydroxyproline in foodstuffs for export. LC-MS/MS method Valid SN/T 3929-2014

PDF similar to SN/T 3929-2014


Standard similar to SN/T 3929-2014

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Basic data

Standard ID SN/T 3929-2014 (SN/T3929-2014)
Description (Translated English) Determination of L-hydroxyproline in foodstuffs for export. LC-MS/MS method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 20,242
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the sample preparation HPLC food for export L- hydroxyproline and - mass spectrometry/mass spectrometry. This standard applies to liquid milk, milk powder, butter, yogurt, butter, cheese, chocolate, milk drinks and other milk and d

SN/T 3929-2014: Determination of L-hydroxyproline in foodstuffs for export. LC-MS/MS method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of L-hydroxyproline in foodstuffs for export. LC-MS/MS method People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of food for export L- hydroxyproline Liquid chromatography - mass spectrometry/mass spectrometry LC-MS/MSmethod Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some content of this document may involve patents, publication of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Shanghai Exit Inspection and Quarantine, the People's Republic of China Exit Inspection and Quarantine and the State Beijing. The main drafters of this standard. Peng Tao, Deng Xiaojun, Zhu Ailing, Zhao Shanzhen, Li Xiaojuan, Wang Jinhua, Individual Students Chen Ying. Determination of food for export L- hydroxyproline Liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard specifies the sample preparation HPLC food for export L- hydroxyproline and - mass spectrometry/mass spectrometry. This standard applies to liquid milk, milk powder, butter, yogurt, butter, cheese, chocolate, milk drinks and other milk and dairy products, protein powder of plant origin Foods L- hydroxyproline liquid chromatography - mass spectrometry/mass spectrometry.

2 Method summary

Sample with concentrated hydrochloric acid hydrolysis at elevated temperature, diluted with water and freeze-dried, redissolved in acetonitrile extraction, graphitized carbon and purified hexane, collected By liquid chromatography - mass spectrometry/mass spectrometry, internal standard.

3 Reagents and materials

Unless otherwise specified, use the analytical reagent, the water is deionized water. 3.1 acetonitrile. HPLC grade. 3.2 formic acid. HPLC grade. 3.3 Ammonium. HPLC grade. 3.4 hexane. HPLC grade. 3.5 hydrochloric acid. AR concentration of 12mol/L. 3.6 acetonitrile saturated with n-hexane. Take 100mL n-hexane (3.4), was added 50mL of acetonitrile (3.1), shaking after mixing, standing layered supernatant spare. 3.7 6mol L hydrochloric acid solution /. Take 50mL hydrochloric acid (3.5) in 100mL volumetric flask, dilute to 100mL with water, set aside. 3.8 Mobile phase A. Weigh 0.158g ammonium formate (3.3) in 400mL of water was added 1mL formic acid (3.2), water to the 500mL, spare. 3.9 Mobile phase B. accurately taken mobile phase A (3.8) 25mL, 475mL acetonitrile mix, spare. 3.10 Standard substances. L- hydroxyproline (L-Hydroxyproline, CAS number. 51-35-4, purity of greater than 99%); 3,4-dehydro - Proline (3,4-Didehydro-L-proline, CAS number. 4043-88-3 purity greater than 99%). 3.11 Standard stock solution. Weigh L- hydroxyproline (3.10) 10mg (accurate to 0.01mg), dissolved in water and dilute to 10mL Brown volumetric flask to prepare a standard stock solution at a concentration of 1.0mg/mL of. 0 ℃ ~ 4 ℃ stored. 3.12 internal standard stock solution. Weigh 3,4 dehydrogenation - proline standard substance (3.10) 10mg (accurate to 0.01mg), dissolved in water Brown and set the volume to 10mL volumetric flask, formulated at a concentration of 1.0mg/mL stock solution of the internal standard. 0 ℃ ~ 4 ℃ stored. 3.13 intermediate standard solution. precise amount of standard stock solution (3.11) 1mL, diluted with water and set the volume to 10mL brown volumetric flask with Intermediate standard solution having a concentration of 100μg/mL of. 0 ℃ ~ 4 ℃ stored. 3.14 Internal standard solution. precise amount of internal standard stock solution (3.12) 1mL, diluted with water and set the volume to 10mL brown volumetric flask to prepare a The concentration of the internal standard solution 100μg/mL of. 0 ℃ ~ 4 ℃ stored. 3.15 sea sand. pure chemistry, particle size 0.65mm ~ 0.85mm, SiO2 content of 99%. 3.16 graphitized carbon. SampliQcarbonSPEBulkSorbent, or equivalent person.

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