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SN/T 2497.3-2019: Test method of import and export dangerous chemicals - Part 3: Daphnia magna reproduction test
Status: Valid SN/T 2497.3: Evolution and historical versions
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| SN/T 2497.3-2019 | English | 399 |
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Test method of import and export dangerous chemicals - Part 3: Daphnia magna reproduction test
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SN/T 2497.3-2019
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| SN/T 2497.3-2010 | English | 679 |
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Test method of import and export dangerous chemicals. Part 3: Daphnia magna reproduction test
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SN/T 2497.3-2010
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Basic data | Standard ID | SN/T 2497.3-2019 (SN/T2497.3-2019) | | Description (Translated English) | Test method of import and export dangerous chemicals - Part 3: Daphnia magna reproduction test | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Word Count Estimation | 19,195 | | Date of Issue | 2019-10-25 | | Date of Implementation | 2020-05-01 | | Older Standard (superseded by this standard) | SN/T 2497.3-2010 | | Regulation (derived from) | General Administration of Customs Announcement No. 166 of 2019 | | Issuing agency(ies) | General Administration of Customs | SN/T 2497.3-2010: Test method of import and export dangerous chemicals. Part 3: Daphnia magna reproduction test
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Test method of import and export dangerous chemicals.Part 3. Daphnia magna reproduction test
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 3. Large Zhuan reproduction test
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This section SN/T 2497 Part 3.
The partial modification of the use of the Organization for Economic Cooperation and Development (OECD) Chemicals testing guidelines NO. 211 (2008) "large-scale reproduction test Zhuan" (English
Version), its technical content with exactly the same method described above, the standard text format according to GB/T 1.1-2000 made editorial changes.
Appendix A of this section is normative appendix, Appendix B, Appendix C, Appendix D, Appendix E, Appendix F for the informative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Jia Xiaochuan, Wangli Bing, Chen training, Lining Tao, Zhang Yuan, Zhang Ying.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 3. Large Zhuan reproduction test
1 Scope
SN/T 2497 provisions of this part of the test equipment import and export of hazardous chemicals major Zhuan propagation tests, test procedures, test quality
Control and test results.
This section applies to the determination of the toxicity of the import and export of hazardous chemicals on aquatic organisms.
2 Terms and definitions
The following terms and definitions apply to this section.
2.1
For breeding females Zhuan beginning of the trial.
2.2
Pro Zhuan Zhuan produced little during the test.
2.3
Parameters measured population growth capacity of the integrated amount of specific species breeding age mortality. Stocks steady state, intrinsic rate of zero.
Population growth, the intrinsic rate is positive; depleted stocks, intrinsic rate is negative.
2.4
Effect Concentration EC x0
Causing the test to reduce the amount of biological reproduction test substance concentration x% of the time.
2.5
Compared with the control of the test organisms was significantly (P < 0.05) the lowest test concentration effects.
2.6
Test set directly below the test substance concentration LOEC, that compared with the control of the test organism is not significant (P < 0.05) effect produced
Setting the highest test substance concentration.
2.7
Zhuan body does not move, that is not able to swim, or Qinghuang test vessel, inside 15s, not observed after abdominal appendage or activities.
3 Test principle
During the large-scale breeding Zhuan determine the effect of the test substance for large Zhuan mortality and reproduction. Large Zhuan containing different concentrations of the test substance
Solution exposure period 21d, by exposure to the test substance in the amount of pro Zhuan breeding compared with control to determine the lowest observed effect concentration
(LOEC) and the no-observed-effect concentration (NOEC), as well as reduce the amount of lead to large-scale breeding Zhuan x% effect concentration.
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