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SN/T 2497.1-2010: Test method of import and export dangerous chemicals. Part 1: Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo
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Test method of import and export dangerous chemicals. Part 1: Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo
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SN/T 2497.1-2010
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Basic data | Standard ID | SN/T 2497.1-2010 (SN/T2497.1-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 1: Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 8,824 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Quoted Standard | OECD 486 | | Adopted Standard | OECD NO.486-1997, MOD | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals mammalian liver cells in vivo unscheduled DNA synthesis (UDS) test terminology and definitions, test theory, test procedures and test results. This standard applies to import and export of dangerous chemicals mammalian liver cells in vivo unscheduled DNA synthesis (UDS) assay. | SN/T 2497.1-2010: Test method of import and export dangerous chemicals. Part 1: Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo
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Test method of import and export dangerous chemicals.Part 1. Unscheduled DNA synthesis (UDS) test with mammalian liver cells in vivo
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 1. mammalian liver cells in vivo unscheduled
DNA synthesis (UDS) test
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
--- Part 14. SDS- polyacrylamide gel electrophoresis.
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This section SN/T 2497 Part 1.
The partial modification of the use of the Organization for Economic Cooperation and Development (OECD) Chemicals testing guidelines NO. 486 (1997) "in vivo mammalian hepatocytes
Outside the cellular DNA synthesis (UDS) test ", the technical content and the method described above exactly the same, in a standard text format press
GB/T 1.1-2000 made editorial changes.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Jia Xiaochuan, Wangli Bing, Lining Tao, Zhang Yuan, Zhao Qing, Xiong Zhongjiang.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 1. mammalian liver cells in vivo unscheduled
DNA synthesis (UDS) test
1 Scope
SN/T 2497 provisions of this part of the import and export of hazardous chemicals in vivo mammalian hepatocyte unscheduled DNA synthesis (UDS) test
Terms and definitions, test principle, test procedures and test results.
This section applies to the import and export of hazardous chemicals in vivo mammalian hepatocyte unscheduled DNA synthesis (UDS) test for detection.
2 Normative references
The following documents contain provisions which, through reference SN/T 2497 to the present, constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
OECD486 mammalian liver cells in vivo unscheduled DNA synthesis (UDS) test
3 Terms and Definitions
OECD486 established as well as the following terms and definitions apply to this section.
3.1
Active quantitative determination, calculation of radiation from the developing UDS UDS test cell nuclear silver grains (NG) is equal to minus nuclear area
The average number of silver particles within the cytoplasm (CG), namely NNG. NG-CG. NNG calculated by the number of cells, and then a parallel culture or cultures
The cell NNG summary.
3.2
Silver Net nuclear grain (NNG) higher than the cells of the laboratory historical negative control values.
3.3
After the removal of DNA containing caused by chemical substances or physical damage to a section of DNA, carried out repair synthesis.
4 Test principle
Mammalian liver cells in vivo unscheduled DNA synthesis (UDS) purpose of the test is to identify infected animals can induce liver cells
DNA repair materials.
In vivo test provides a research chemical liver toxicity effect of genetic methods, the end of the test showed that the liver cells DNA
Damage and Repair ensued. The liver is the main organ of the body's metabolism of chemicals absorbed, so the liver is the in vivo detection of DNA damage
Suitable injury site.
After the chemical or physical factors induce DNA damage, cell unscheduled DNA synthesis start procedure to excise or remove DNA damage in area
Domain, UDS test in vivo mammalian liver cells is to detect damaged DNA repair synthesis. Liver cell cycle in the S phase frequency
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