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SN/T 1943-2019: Protocol of PCR and real-time PCR for qualitative testing genetically modified components in transgenic wheat and its processed products
Status: Valid SN/T 1943: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1943-2019 | English | 259 |
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Protocol of PCR and real-time PCR for qualitative testing genetically modified components in transgenic wheat and its processed products
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SN/T 1943-2019
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| SN/T 1943-2007 | English | 439 |
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Protocol of polymerase chain reaction and real-time PCR for qualitative detecting genetically modified components in transgenic wheat
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SN/T 1943-2007
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Basic data | Standard ID | SN/T 1943-2019 (SN/T1943-2019) | | Description (Translated English) | Protocol of PCR and real-time PCR for qualitative testing genetically modified components in transgenic wheat and its processed products | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B22 | | Word Count Estimation | 12,160 | | Date of Issue | 2019-10-25 | | Date of Implementation | 2020-05-01 | | Older Standard (superseded by this standard) | SN/T 1943-2007 | | Regulation (derived from) | General Administration of Customs Announcement No. 166 of 2019 | | Issuing agency(ies) | General Administration of Customs | SN/T 1943-2007: Protocol of polymerase chain reaction and real-time PCR for qualitative detecting genetically modified components in transgenic wheat
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Protocol of polymerase chain reaction and real-time PCR for qualitative detecting genetically modified components in transgenic wheat
Exit inspection and quarantine industry standard book People's Republic of China
PCR and genetically modified wheat
Real-time PCR for qualitative detection method
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Heilongjiang Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Luan Feng Xia, Zhang Hongxiang, White Moon, Gao Yong, Huang Wensheng, Xu Baoliang.
This standard such as those involving national has applied for patents, patent holders to renounce the use of Chinese territory.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
PCR and real-time PCR qualitative detection method for genetically modified wheat
1 Scope
This standard specifies PCR and real-time PCR detection of genetically modified wheat.
This standard applies to wheat genetically modified PCR and real-time PCR for detection.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. Where dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods
SN/T 1193 genetic testing laboratory technical requirements
SN/T 1194 to detect genetically modified plants and their products sampling and sample preparation methods
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this standard.
3.1.1
The species itself does not have, from the functional DNA sequences of other species imported by means of molecular biology, it was in the
Kinds of expression, so that the species to acquire new property characteristics.
3.1.2
It does not have its own species, but from functional gene sequences from other species.
3.1.3
Transgenic crop genome copy number constant, allelic variations, in other crop species not present in the gene is not displayed.
The gene can be used to evaluate whether the presence of crop species in the sample, but also on the quantitative detection of a target gene as reference gene sequence
Measured, but can also determine the success of DNA extraction, and verify the presence of inhibitory substances PCR reaction system.
3.1.4
Use of other bio-genetic bioengineering technology transferred, so that the performance of the new biological species biological traits.
3.2 Acronyms
The following abbreviations apply to this standard.
3.2.2 Ct. cyclethreshold, each reaction tube fluorescent signal reaches the set threshold number of cycles experienced.
3.2.3 GAG56D. triticmaestivumpartialGAG56Dgeneforgamma-gliadin, wheat gliadin gene (Gene-
? Bank Number. GI. 10638295). Wheat genus-specific endogenous gene.
3.2.4 NAC. noamplificationcontrol, negative control.
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