SN/T 1151.5: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1151.5-2015 | English | RFQ |
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3 days [Need to translate]
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(Technical specifications of shrimp baculovirus disease quarantine)
| Valid |
SN/T 1151.5-2015
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| SN/T 1151.5-2014 | English | 399 |
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3 days [Need to translate]
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Quarantine protocol for tetrahedral baculovirosis
| Obsolete |
SN/T 1151.5-2014
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| SN/T 1151.5-2003 | English | 279 |
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3 days [Need to translate]
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The shrimp baculovirus (BP) detection method
| Obsolete |
SN/T 1151.5-2003
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PDF similar to SN/T 1151.5-2015
Basic data | Standard ID | SN/T 1151.5-2015 (SN/T1151.5-2015) | | Description (Translated English) | (Technical specifications of shrimp baculovirus disease quarantine) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B51 | | Classification of International Standard | 67.120.30 | | Date of Issue | 2015-02-09 | | Date of Implementation | 2015-09-01 | | Older Standard (superseded by this standard) | SN/T 1151.5-2003 | | Regulation (derived from) | State-Quality-Inspection-Accreditation [2015] 59 | | Issuing agency(ies) | General Administration of Customs | SN/T 1151.5-2014: Quarantine protocol for tetrahedral baculovirosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specifications shrimp baculovirus disease quarantine)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1151.5-2003
Technical specifications prawn baculovirus disease quarantine
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1151 is divided into six sections.
--- Taura Syndrome shrimp quarantine technical specifications;
--- White spot disease quarantine technical specifications;
--- Monodon baculovirus (MBV) diagnostic methods;
--- Shrimp yellow head disease quarantine technical specifications;
--- Prawn baculovirus disease quarantine technical specifications;
--- White Spot virus dot blot and in situ hybridization procedures.
This section SN/T 1151 Part 5.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The partial replacement of SN/T 1151.5-2003 "shrimp baculovirus (BP) detection method."
This section compared with the SN/T 1151.5-2003, the main technical changes are as follows.
--- Increased sampling;
--- Primers for PCR test has been modified;
--- Amplified product increases the reference sequence.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, the Chinese people
Republic of Shandong CIQ.
The main drafters of this section. Shixiu Jie, He Junjiang, Jiang Yuting Wang Jinjin, in force, Zhengxiao Cong, Jia Peng, blue Wensheng, Yang Jinshun, Liu Orientin.
This part of the standard replaces the previous editions are.
--- SN/T 1151.5-2003.
Technical specifications prawn baculovirus disease quarantine
1 Scope
SN/T 1151 to the present standard specifies the shrimp baculovirus microscopy, polymerase chain reaction detection method.
This standard applies to prawn baculovirus disease epidemiology, diagnosis, quarantine and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
GB 19489 General requirements for laboratory biosafety
3 Reagents and materials
3.1 Water. in line with GB/T 6682 a water specifications.
3.2 TaqDNA polymerase. -20 ℃ to save, not to repeated freezing and thawing or extreme temperature changes.
3.3 dNTPs. containing dCTP, dGTP, dATP, dTTP each 10mmol/L.
3.4 primer. a pair of primers, concentration of 40μmol/L, the sequence is.
6581F. 5'-TGT-AGC-AGC-AGA-GAA-GAG-3 '
6582R. 5'-CAC-TAA-GCC-TAT-CTC-CAG-3 '
Amplification of viral genes in the DNA fragment of 645bp.
3.5 ethanol. AR, before using the pre-cooled to -20 ℃.
4 instruments and equipment
4.1 scissors and tweezers.
4.2 slides and cover slips.
4.3 incubator.
4.4 ordinary refrigerator and ultra-low temperature freezer.
4.5 upright microscopes.
4.6 centrifuges and centrifuge tubes.
4.7 micropipette and tips.
4.8 PCR amplification.
4.9 electrophoresis.
4.10 UV lamp observation or gel imager.
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