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SN/T 1151.2-2011: Quarantine protocol for white spot disease
Status: Valid SN/T 1151.2: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1151.2-2011 | English | 369 |
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Quarantine protocol for white spot disease
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SN/T 1151.2-2011
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| SN/T 1151.2-2002 | English | 279 |
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Test method of polymerase chain reaction (PCR) for white spot virus (WSV)
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SN/T 1151.2-2002
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PDF similar to SN/T 1151.2-2011
Basic data | Standard ID | SN/T 1151.2-2011 (SN/T1151.2-2011) | | Description (Translated English) | Quarantine protocol for white spot disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 14,199 | | Date of Issue | 2011-05-31 | | Date of Implementation | 2011-12-01 | | Older Standard (superseded by this standard) | SN/T 1151.2-2002; SN/T 1151.6-2004 | | Quoted Standard | GB/T 6682; GB/T 18088 | | Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 291 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the shrimp white spot disease PCR, dot blot and in situ hybridization detection methods. This section applies to shrimp white spot disease epidemiology, monitoring, diagnosis and quarantine. | SN/T 1151.2-2011: Quarantine protocol for white spot disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for white spot disease
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1151.2-2002, SN/T 1151.6-2004
White spot disease quarantine Technical Specifications
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1151 series of standards is divided into six parts.
--- Taura Syndrome shrimp quarantine Technical Specifications
--- White spot disease quarantine Technical Specifications
--- Monodon baculovirus (MBV) Diagnosis
--- Shrimp yellow head disease quarantine Technical Specifications
--- Shrimp baculovirus (BP) test method
--- White Spot virus dot blot and in situ hybridization procedures
This section SN/T 1151 Part 2.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The partial replacement of SN/T 1151.2-2002 "of white spot virus (WSV) polymerase chain reaction (PCR) detection method" and
SN/T 1151.6-2004 "White Spot virus dot blot and in situ hybridization procedures."
This standard compared with SN/T 1151.2-2002 and SN/T 1151.6-2004, the main technical changes are as follows.
Increased clinical symptoms and comprehensive determination method.
This part reference OIE (OIE) "aquatic animal disease diagnostic manual" (2009 edition) in chapter 2.2.5.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China SZCIQ, Chinese Academy of Fishery Sciences Yellow Sea Fisheries Research Institute,
People's Republic of China Shandong CIQ.
The main drafters of this part. He Junqiang, Orientin Liu, Huang Jie, Yang Bing, Ray quality text, Yue Zhiqin, high Lung Ying, Jiang Yu-lin, Shixiu Jie.
This part of the standard replaces the previous editions are.
--- SN/T 1151.2-2002;
--- SN/T 1151.6-2004.
White spot disease quarantine Technical Specifications
1 Scope
SN/T 1151 This section provides the shrimp white spot disease detection PCR, dot blot and in situ hybridization.
This section applies to White spot disease in epidemiological investigation, monitoring, diagnosis and quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Abbreviations
The following abbreviations apply to this document
WSD (whitespotdisease). White Spot Disease
WSV (whitespotvirus). White Spot Virus
PCR (polymerasechainreaction). Polymerase chain reaction
NBT (nitrobluetetrazolium). NBT
BCIP (5-bromo-4chloro-3-indoyl). 5- bromo-4-chloro-3-indolyl phosphate - toluidine salt
DMF (dimethylformamide). dimethylformamide
4 Overview
WSD is WSV caused by a contagious disease of shrimp, shrimp farming is the world's most serious hazards shrimp virus disease,
It has been included in the World Organisation for Animal Health (OIE) Aquatic Animal Diseases list, and was included in the list of a class of animal diseases. Virion
From the rod to the oval-shaped, with three capsule, larger particles (80nm ~ 120nm × 250nm ~ 380nm). Genome size is 290kb.
It can produce WSV characteristic inclusions within the infected cell nuclei hypertrophy.
5 reagent material and equipment
This standard reagents used if no special instructions are analytical reagents. Test water line with GB/T 6682 a water requirements.
5.1 Taq enzyme. -20 ℃, avoid repeated freezing and thawing or extreme temperature changes.
5.2 dNTP. dCTP, dGTP, dATP, dTTP each 10mmoL/L.
5.3 primer. Two pairs of primers concentration 40μmoL/L, which sequences are as follows.
146F1. 5'-ACTACTAACTTCAGCCTATCTAG-3 '
146R1. 5'-TAATGCGGGTGTAATGTTCTTACGA-3 '
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