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SN/T 1151.1-2011: Quarantine protocol for shrimp taura syndrome
Status: Valid SN/T 1151.1: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1151.1-2011 | English | 259 |
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Quarantine protocol for shrimp taura syndrome
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SN/T 1151.1-2011
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| SN/T 1151.1-2002 | English | 239 |
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Test method of reverse transcription polymerase chain reaction (RT-PCR) for tuara syndrome virus (TSV)
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SN/T 1151.1-2002
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PDF similar to SN/T 1151.1-2011
Basic data | Standard ID | SN/T 1151.1-2011 (SN/T1151.1-2011) | | Description (Translated English) | Quarantine protocol for shrimp taura syndrome | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 10,116 | | Date of Issue | 2011-05-31 | | Date of Implementation | 2011-12-01 | | Older Standard (superseded by this standard) | SN/T 1151.1-2002 | | Quoted Standard | GB/T 18088 | | Adopted Standard | OIE Aquatic Animal Disease Diagnostic Manual 2009, MOD | | Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 291 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of operation of shrimp Taura syndrome RT-PCR and Real-time RT-PCR detection. This section applies to the epidemiological investigation of shrimp Taura syndrome, diagnosis, quarantine and monitoring. | SN/T 1151.1-2011: Quarantine protocol for shrimp taura syndrome---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for shrimp taura syndrome
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1151.1-2002
Taura Syndrome shrimp quarantine Technical Specifications
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1151 series of standards is divided into six parts.
--- Taura Syndrome shrimp quarantine technical specifications;
--- White spot disease quarantine technical specifications;
--- Monodon baculovirus (MBV) diagnostic methods;
--- Shrimp yellow head disease quarantine technical specifications;
--- Shrimp baculovirus (BP) test methods;
--- White Spot virus dot blot and in situ hybridization procedures.
This section SN/T 1151 Part 1.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The partial replacement of SN/T 1151.1-2002 "shrimp Taura syndrome virus (TSV) reverse transcription polymerase chain reaction (RT-
PCR) diagnostic methods. " This section compared with the SN/T 1151.1-2002, major technical changes as follows.
--- Integration and optimization of RT-PCR detection method;
--- New Real-timeRT-PCR detection method.
The partial modification using World Organisation for Animal Health (OIE) of "aquatic animal disease diagnostic manual" (2009 Edition) chapter 2.2.4, and
Taura Syndrome shrimp by RT-PCR method for the integration and optimization.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this section. Xiong Wei, Zhang Qiang, Liu Orientin, Cheng Tang, Jing Jiang, Li Jian, Qiu Lu, Wang Qiao whole, Huang Zhongrong, Hu Yongqiang.
This part of the standard replaces the previous editions are.
--- SN/T 1151.1-2002.
Taura Syndrome shrimp quarantine Technical Specifications
1 Scope
SN/T 1151 This section specifies the method of operation of shrimp Taura syndrome RT-PCR and Real-timeRT-PCR detection.
This section applies to epidemiology, diagnosis, and monitoring of quarantine shrimp Taura syndrome.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Overview
Taura syndrome (Taurasyndrome) is an infection by the Taura syndrome virus (Taurasyndromevirus, TSV) caused
A serious threat to shrimp farming important disease, which is the World Organisation for Animal Health (OIE) prescribed notifiable aquatic animal diseases. TSV few
It can infect virtually all types of farmed shrimp, such as. South American white shrimp, red forehead shrimp, brown shrimp, white shrimp, black tiger shrimp, etc., and the incubation period of the infection
Shrimp, its death rate. TSV infection has three distinct phases. acute, transition and chronic phase. In the acute phase, shrimp skin epithelium
Tissue sections can be seen in the typical pathological lesions. In the transition period and the chronic phase, but not typical pathological injury; shrimp main incidence of death
To occur in the acute phase, the survivors after acute infection after a brief transition period after entering a long period of chronic infection, in a slow sexy
Transfection of lymphoid organs shrimp in their lifetime presence of the virus, the virus carrying the shrimp can spread the virus to other susceptible shrimp level. disease
Toxicity studies confirm, TSV is a small icosahedral non-enveloped viruses, the diameter of 32nm, its genome is a single positive-strand RNA by the 10205
Nucleotides, containing two large open reading frame (Openreadframe, ORF), ORF1 encodes non-structural proteins such as helicase,
Protease and RNA-dependent RNA polymerase, etc., ORF2 encoding structural proteins such as capsid protein VP1, VP2, VP3 (large molecular weight
Small were 55kDa, 40kDa, 24kDa).
4 Reagents and materials
4.1 Taq Taq polymerase enzyme 10-fold concentrated buffer. Taq enzyme concentration 5U/μL, -20 ℃, avoid repeated freezing and thawing.
4.2 retroviral reverse transcriptase enzyme 10-fold concentrated buffer. reverse transcriptase concentration of 50U/μL, -20 ℃, avoid repeated freezing and thawing.
4.3 RNA inhibitors. a concentration of 40U/μL, -20 ℃, avoid repeated freezing and thawing.
4.4 dNTP. containing dATP, dGTP, dCTP, dTTP each 10mmol/L, -20 ℃ preservation.
4.5 RT-PCR primer. primer concentration were 20μmol/L; there are two pairs of primers can optionally use one; as primers 9992F and 9195R
OIE Recommended primers amplified gene fragment 231 bp in size, gene amplification primers TSVF TSVR and size of 295bp,
Its sequence is as follows.
The upstream primer (9992F). 5'-AAG-TAG-ACA-GCC-GCG-CTT-3 '
Downstream primer (9195R). 5'-TCA-ATG-AGA-GCT-TGG-TCC-3 '
The upstream primer (TSVF). 5'-ATT-GAA-TAC-TTA-GCA-CAG-CGA-CC-3 '
Downstream primer (TSVR). 5'-GAA-CAC-CAC-TAC-CAT-AGG-GGA-G-3 '
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