SN/T 1088: Evolution and historical versions
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| SN/T 1088-2024 | English | RFQ |
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3 days [Need to translate]
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(Technical specifications for quarantine of brucellosis)
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SN/T 1088-2024
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| SN/T 1088-2010 | English | 1059 |
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Quarantine Frotocol for Brucellosis
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SN/T 1088-2010
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| SN/T 1088-2002 | English | 199 |
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Procedure of Plate Agglutination Test for Brucellosis
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SN/T 1088-2002
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PDF similar to SN/T 1088-2024
Basic data | Standard ID | SN/T 1088-2024 (SN/T1088-2024) | | Description (Translated English) | (Technical specifications for quarantine of brucellosis) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Date of Issue | 2024-12-31 | | Date of Implementation | 2025-07-01 | | Issuing agency(ies) | General Administration of Customs | SN/T 1088-2010: Quarantine Frotocol for Brucellosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine Frotocol for Brucellosis
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1088-2002, SN/T 1089-2002, SN/T 1090-2002,
SN/T 1394-2004, SN/T 1525-2005
Brucellosis quarantine Technical Specifications
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Table of Contents
Preface Ⅰ
1 Scope 1
2 Normative references 1
3 1 Overview
4 1 clinical symptoms and pathological changes
5 Pathogen Identification
5.1 Microscopic examination 1
5.2 bacterial culture 2
5.3 Polymerase chain reaction (PCR) 3
6 serologic testing 5
6.1 bengal plate agglutination test 5
6.2 buffered plate agglutination test 6
6.3 tube agglutination test 7
6.4 complement fixation test 9
6.5 ELISA 16
6.6 Fluorescence Polarization Test 17
6.7 whole milk ring test 18
7 total judgment 18
Appendix A (informative) Overview 19
Annex B (informative) clinical symptoms and pathological changes 20
Annex C (normative) reagent preparation 21
Annex D (informative) 25 international units of agglutination
Hemolysin titer determination Appendix E (normative) 26
Annex F (informative) Calculation of complement titer 27
Determination of Annex G (informative) antigen titer of 28
Appendix H (informative) international complement fixation unit 29
Annex I (informative) hemolysin titer determination 30
Appendix J (informative) complement titer determination 31
Determination of Annex K (normative) antigen titer table 32
Formulated Appendix L (normative) standard hemolysis hole 33
Annex M (informative) indirect ELISA 34
Annex N (informative) Competitive ELISA 37
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1088-2002 "brucellosis plate agglutination test procedures", SN/T 1090-2002 "brucellosis
Disease tube agglutination test procedures ", SN/T 1089-2002" brucellosis complement fixation test procedures ", SN/T 1394-
2004 "brucellosis whole milk ring test methods", SN/T 1525-2005 "trace brucellosis complement fixation test methods."
In addition to this standard editorial changes, the major technical changes are as follows.
--- Increased brucellosis microscopic examination;
--- Increased bacterial culture;
--- Increasing the polymerase chain reaction;
--- Increasing the buffer plate agglutination test;
--- Increasing the enzyme-linked immunosorbent assay;
--- Increased fluorescence polarization test.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Wang Yuling, Liu Pei, Zhu Shiqiang, Hou Yanmei, Luan Shen Shun.
This standard replaces the standards previously issued as follows.
--- SN/T 1088-2002;
--- SN/T 1090-2002;
--- SN/T 1089-2002;
--- SN/T 1394-2004;
--- SN/T 1525-2005.
Brucellosis quarantine Technical Specifications
1 Scope
This standard specifies the brucellosis microscopic examination, bacterial culture, polymerase chain reaction, bengal plate agglutination test, buffered plate agglutination test
Test, tube agglutination test, complement fixation test, enzyme-linked immunosorbent assay, fluorescence polarization test, whole milk ring test and other technical requirements.
This standard applies to cattle, pigs brucellosis quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1942.1 and export foodstuffs of animal origin Brucella test methods - Part 1. Separation and counting method
3 Overview
See Appendix A.
4 clinical symptoms and pathological changes
See Appendix B.
5 pathogen identification
5.1 Microscopy
5.1.1 Reagents
Crystal violet staining solution, Gram iodine, sand yellow counterstain, carbolic acid fuchsin solution, 3% hydrochloric acid and alcohol, alkaline methylene blue staining solution, method of preparation
See Appendix C.
5.1.2 Major equipment
microscope.
5.1.3 Test Procedure
5.1.3.1 Sample Collection
Take appropriate amount of tissue or fluid sample to be tested, filled in a vial for inspection.
5.1.3.2 Sample Preparation
Take appropriate samples to be tested with distilled water and mix with 4 layers of gauze filtered and the filtrate 5000r/min centrifugal 5min, with precipitation smears; also
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