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SN/T 1086-2011: Quarantine protocol for bovine genital campylobacteriosis
Status: Valid SN/T 1086: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1086-2011 | English | 369 |
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Quarantine protocol for bovine genital campylobacteriosis
| Valid |
SN/T 1086-2011
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| SN/T 1086-2002 | English | 359 |
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The isolation and identification of the fetus curved bacilli
| Obsolete |
SN/T 1086-2002
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PDF similar to SN/T 1086-2011
Basic data | Standard ID | SN/T 1086-2011 (SN/T1086-2011) | | Description (Translated English) | Quarantine protocol for bovine genital campylobacteriosis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 14,159 | | Date of Issue | 2011-05-31 | | Date of Implementation | 2011-12-01 | | Older Standard (superseded by this standard) | SN/T 1086-2002 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 291 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the bovine reproductive tract campylobacteriosis quarantine technical requirements. This standard applies to bovine reproductive tract pathogen Campylobacter isolation and identification, and can be used for disease quarantine, diagnostic and epidemiological investigation. | SN/T 1086-2011: Quarantine protocol for bovine genital campylobacteriosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for bovine genital campylobacteriosis
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1086-2002
Bovine genital campylobacteriosis quarantine Technical Specifications
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1086-2002 "isolation and identification of Campylobacter fetus."
This standard compared with SN/T 1086-2002, the main technical changes are as follows.
--- Modify the standard name;
--- Increased indirect fluorescent antibody test and PCR detection method.
The standard reference to the OIE (OIE) "Terrestrial Animals Manual of diagnostic tests and vaccines (mammals, birds and bees)"
(2009 Edition) (ManualofDiagnosticTestsandVaccinesforTerrestrialAnimals2009) 2.4.5 chapter.
The main changes are as follows.
--- This standard adopts the pathogen isolation and identification OIE regulations, indirect fluorescent antibody detection methods, PCR detection methods;
--- This standard deleted OIE mentioned ELISA method.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Hou Yanmei, Guo Fusheng, Qin Zhen Kui, Huo Lei, Shen Luan Shun.
This standard replaces the standards previously issued as follows.
--- SN/T 1086-2002.
Bovine genital campylobacteriosis quarantine Technical Specifications
1 Scope
This standard specifies the bovine genital campylobacteriosis quarantine technical requirements.
This standard applies to bovine reproductive tract Campylobacter Isolation and identification of pathogen, the disease can be used for quarantine, diagnosis and epidemiological investigation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
TEM. enrichment transport medium
CH. cysteine beef heart extract medium
MH. MulerHinton agar
4 Equipment and Materials
4.1 Mixed gas (mass ratio of 3.5% O2,10% CO2,86.5% N2).
4.2 anaerobic tank.
4.3 Sampling rods and suction ball.
4.4 gel imaging system.
5 medium and reagents
See Appendix A. Test water should comply with GB/T 6682 requirements.
6 Isolation and Identification
6.1 Sample Collection
6.1.1 bull semen and smegma
After decontamination bull urethra, the sampling rod inserted into the female end of the sampling tube and the other end of the suction ball flattened and twitching back and forth, drawing package
Skin was removed quickly inserted sampling wand 3mLPBS filled with liquid after absorbing several times blowing vial immediately sealed.
When the foreskin flush with 20mL ~ 30mLPBS was injected into the foreskin capsule, hard friction 15s ~ 20s after the injection of the liquid collected
Sterile bottle, immediately sealed; Smegma can also be collected from fake vagina after semen collection, which was washed with 20mL ~ 30mLPBS fake vagina
obtain.
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