GB/T 33681: Evolution and historical versions
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(Pretreatment of high throughput gene sequencing samples -- Part 1: Pretreatment of animal tissue samples)
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GB/T 33681-2017
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Methods to prepare samples for high-throughput gene sequencing -- Part 1: Preparing samples of animal tissues
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GB/T 33681.1-2017
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Basic data | Standard ID | GB/T 33681-2017 (GB/T33681-2017) | | Description (Translated English) | (Pretreatment of high throughput gene sequencing samples -- Part 1: Pretreatment of animal tissue samples) | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Date of Issue | 2017-05-12 | | Date of Implementation | 2017-12-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33681.1-2017: Methods to prepare samples for high-throughput gene sequencing -- Part 1: Preparing samples of animal tissues
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Methods to prepare samples for high-throughput gene sequencing - Part 1. Preparing samples of animal tissues
ICS 07.080
A40
National Standards of People's Republic of China
Pretreatment of high throughput gene sequencing samples
Part 1. Animal tissue sample pretreatment
Part 1. Preparingamplesofanimaltissues
2017-05-12 released
2017-12-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Directory
Preface I
1 Scope 1
2 normative reference document 1
3 Terms and definitions 1
4 acronym 2
5 General 2
6 laboratory requirements 3
Main equipment and equipment 3
8 Test Step 3
Quality assessment 4
Appendix A (informative) Extraction of nucleic acids 5
Appendix B (informative) Quality evaluation 6
Appendix C (normative appendix) Laboratory requirements 7
Appendix D (informative) Fragmentation 11
Appendix E (informative) Single molecule amplification 13
Reference 14
Foreword
GB/T 33681 "high-throughput gene sequencing sample pretreatment method" is divided into three parts.
Part 1 Animal tissue sample pretreatment;
Part 2 Plant Tissue Sample Pretreatment;
Part 3 Microorganism Tissue Sample Pretreatment.
This part is part 1 of GB/T 33681.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
This part of the National Biochemical Testing Standardization Technical Committee (SAC/TC387) proposed and centralized.
This part of the drafting unit. Shenzhen Hua Kang Gene Technology Co., Ltd., Shenzhen Hua Kang high-throughput biotechnology research institute, Guangzhou Kang Xin
Ruike Health Technology Co., Ltd., Wuhan Kang Xinrui Gene Health Technology Co., Ltd., China Testing Technology Research Institute, Shanghai Jiaotong University
College Affiliated Ruijin Hospital, National Human Genome Research Center of the South, Zhongshan University Cancer Center.
The main drafters of this part. Sheng Sitong, Tan Hui Biao, Li Hua, Zeng Tao, Yang Jiebin, Zhou Lihua, Zhang Jun, Wang Shengyue, Chen Gong.
Pretreatment of high throughput gene sequencing samples
Part 1. Animal tissue sample pretreatment
1 Scope
This part of GB/T 33681 specifies the technique for pre-processing samples of animal tissue samples to be tested in high-throughput gene sequencing techniques
Claim.
This part is suitable for the pretreatment of samples of animal tissues to be tested during high-throughput gene sequencing.
This part applies to blood, epidermal swab samples, fresh animal tissue samples (muscles, organs, hair, bones), formaldehyde fixed animal tissue
Sample, paraffin embedded animal tissue samples.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 26798 single - beam UV - Vis spectrophotometer
Dual - beam UV - Vis spectrophotometer GB/T 26813
GB/T 27025 General requirements for testing and calibration laboratory capabilities
Technical specification for high - throughput gene sequencing GB/T
JJG646 pipette
YY0569 Ⅱ level biological safety cabinet
YY/T 0657 Medical centrifuge
Medical institutions, clinical gene amplification management approach
ASTME1342 Preserves bacteria, fungi, protozoa, viruses, genetic elements and animals by freezing, freeze-drying and cryopreservation
Standards and regulations for plant and plant tissue (Standardpracticeforpreservationbyfreezing, freeze-drying, andlow
temperaturemaintenanceofbacteria, fungi, protista, viruses, geneticelements, andanimalandplant
tissues
3 terms and definitions
GB/T 30989 Definitions and the following terms and definitions apply to this document.
3.1
Animal tissue sample animaltissuesample
An animal tissue material that provides DNA or RNA for constructing a sequencing library.
3.2
Connector adapter
A fragment of a synthetic length that is capable of matching (concatenating) a blunt end or a cohesive end.
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