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GB/T 33682-2025: General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
Status: Valid GB/T 33682: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 33682-2025 | English | 199 |
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General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
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GB/T 33682-2025
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| GB/T 33682-2017 | English | 109 |
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General microorganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
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GB/T 33682-2017
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PDF similar to GB/T 33682-2025
Basic data | Standard ID | GB/T 33682-2025 (GB/T33682-2025) | | Description (Translated English) | General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 9,935 | | Date of Issue | 2025-05-30 | | Date of Implementation | 2025-09-01 | | Older Standard (superseded by this standard) | GB/T 33682-2017 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 33682-2025: General microoganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 07.080
CCSA40
National Standard of the People's Republic of China
Replaces GB/T 33682-2017
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
General principles for methods of identification of microorganisms
Released on 2025-05-30
2025-09-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 33682-2017 "General rules for identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry" and
Compared with GB/T 33682-2017, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
--- Added requirements for pre-treatment methods for different strains (see 8.2);
--- Added instrument setting condition requirements (see 8.4.1);
--- Added "quality control" requirements (see 8.6);
--- Changed the interpretation criteria of “result interpretation” (see 8.8, 8.7 of the.2017 edition);
--- Changed the reporting requirements for “Results Report” (Chapter 9, Chapter 9 of the.2017 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Technical Committee for Biochemical Testing Standardization (SAC/TC387).
This document was drafted by. Hebei Provincial Food Inspection Institute, China Testing Technology Research Institute, Heilongjiang Provincial Intellectual Property Protection Center, Tsinghua University
University, Guangzhou Huaxin Testing Technology Co., Ltd., Suzhou Shipu Testing Technology Co., Ltd., Henan University, Beijing Institute of Food Science, Nanjing
University of Finance and Economics, Hebei Medical University, Beijing Technology and Business University, Zhejiang University of Technology, China Institute of Inspection and Quarantine, Lanzhou Baiyuan Gene Technology
Ltd.
The main drafters of this document are. Zhang Yan, Zhou Wei, Wang Yi, Zhou Lihua, Peng Liping, Li Yongbo, Kang Wenyi, Sun Yong, Lv Pin, Wendi, Fan Sufang,
Tang Xiaozhi, Feng Xiao, Zhang Yalun, Wang Yanbo, Zhou Xuxia, Zhang Rui, Che Tuanjie, Chen Chen, Liao Huayong, Dai Dan, Wang Hui, Liu Shuai, Ren Xiaoyan, Niu Xiangtao,
Zou Yan and Zheng Xiaoling.
The previous versions of this document and the documents it replaces are as follows.
---First published in.2017 as GB/T 33682-2017;
---This is the first revision.
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
General principles for methods of identification of microorganisms
1 Scope
This document specifies the general method for identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
This document applies to the matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of microbial species.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489 General requirements for laboratory biosafety
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
The isolated and purified single colony is directly applied to the target plate, and after drying, the matrix is added and mixed. The microbial soluble protein and the matrix form
After forming co-crystals, mass spectrometry fingerprints of protein molecules with different mass-to-charge ratios were obtained through a vacuum time-of-flight tube.
4 Abbreviations
The following abbreviations apply to this document.
BTS. Bacteria Detection Standard Sample.
MALDI-TOFMS. matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
MALDI-TOF-TOFMS. matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry.
MSP. main spectrum plot.
5 Principle
Common microorganisms are composed of special proteins that are different from other species, and therefore have unique protein fingerprints, which are determined by species.
It is determined by the genetic characteristics and is less affected by external environmental conditions.
Samples (from clinical samples such as sputum, blood, body fluids, swabs, and food, cosmetics, medicines, and the environment, etc.) are properly processed and cultured in solid
After culturing and isolating a single colony on the matrix, the sample was directly spotted onto the target plate and placed into the matrix-assisted laser desorption ionization time-of-flight mass spectrometer.
Ionization is separated according to the mass/charge (m/z) ratio, and the collected data is compared with the microbial fingerprint library for identification.
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