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GB/T 29429-2012 English PDF

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GB/T 29429-2012: Detection and identification of Xanthomonas fragariae Kennedy and King
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 29429-2012	English	259	Add to Cart	3 days [Need to translate]	Detection and identification of Xanthomonas fragariae Kennedy and King	Valid	GB/T 29429-2012

PDF similar to GB/T 29429-2012

Standard similar to GB/T 29429-2012

GB/T 43165 GB/T 40135 GB/T 19495.4 GB/T 31790 GB/T 29431 GB/T 29430

Basic data

Standard ID	GB/T 29429-2012 (GB/T29429-2012)
Description (Translated English)	Detection and identification of Xanthomonas fragariae Kennedy and King
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B16
Classification of International Standard	65.020.01
Word Count Estimation	11,188
Quoted Standard	GB/T 4789.28-2003; SN/T 1157
Regulation (derived from)	National Standards Bulletin 2012 No. 42
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary	This standard specifies the strawberry angular leaf spot fungus Xanthomonas fragariae Kennedy & King of quarantine and identification by morphological characteristics, biological characteristics and specificity of PCR reactions as the basis, a clear sampl

GB/T 29429-2012: Detection and identification of Xanthomonas fragariae Kennedy and King

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xanthomonas fragariae Kennedy ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification of strawberry angular leaf spot fungus Issued on. 2012-12-31 2013-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shandong Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Liu Tianhong, Shaoxiu Ling, Zhao Wenjun, Yang Wan wind, Li Yan, Wei Hau, Sha days Hui, Meng Xianglong. Quarantine and identification of strawberry angular leaf spot fungus

1 Scope

This standard specifies the strawberry angular leaf spot fungus XanthomonasfragariaeKennedy PCR reaction specificity and biological characteristics as the basis, a clear sample collection, pathogen isolation, pathogenicity assay, PCR reaction, the sample protection The method stored. This standard applies to quarantine and identification of strawberry seedlings in strawberry angular leaf spot fungus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 4789.28-2003 Microbiological examination of food hygiene staining, media and reagents SN/T 1157 export plant seedlings quarantine rules

3 Basic information of strawberry angular leaf spot fungus

Chinese name. strawberry angular leaf spot fungus. Name. XanthomonasfragariaeKennedy Disease English. Angularleafspotofstrawberry. Belonging Prokaryotae Procaryote, thin-walled bacteria door Gracilicutes, dark bacteria Gang Scotobacteria, Pseudomonas Branch Pseudomonadaceae, Xanthomonas genus Xanthomonas. The bacteria can splash through rainwater for irrigation and in the field of local spread, spread, disease-carrying and inclusions in Strawberry Strawberry in disease Debris may trade with a short distance and long-distance transport of propagation. For additional information, see Appendix A. strawberry angular leaf spot fungus

4 principle of the method

According strawberry angular leaf spot fungus damage symptoms of sample collection, laboratory isolation, culture and pathogenicity determination, as well as molecular biology test Given other methods, based on the symptoms of disease, pathogen morphological characteristics, biological characteristics, biochemical characteristics, pathogenicity assay, and molecular biology special Sign determination.

5 apparatus and appliances

Biological microscopes, incubator, autoclave, electronic balance (inductance 0.01mg), Shaking Incubator, clean benches, PCR, electrophoresis apparatus, heated water bath, high-speed refrigerated centrifuge, pipettes, pipette tips, mortar, measuring beakers, petri dishes, PCR Pipe, tube, vaccination needles, glass, scissors and so on.

6 Reagents

Peptone, yeast extract, sucrose, dextrose, agar, glacial acetic acid, ethanol, ethylenediaminetetraacetic acid (EDTA), cetyltrimethylammonium bromide

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