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GB/T 29431-2012: Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.
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| GB/T 29431-2012 | English | 279 |
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Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.
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GB/T 29431-2012
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Basic data | Standard ID | GB/T 29431-2012 (GB/T29431-2012) | | Description (Translated English) | Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al. | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 12,135 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | National Standards Bulletin 2012 No. 42 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the tomato canker quarantine identification. This standard applies to tomato seeds, seedlings and other related materials Solanaceae tomato canker quarantine and identification. | GB/T 29431-2012: Detection and identification of Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.
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Detection and identification of Clavibacter michiganensis subsp.michiganensis (Smith) Davis et al.
ICS 65.020.01
B16
National Standards of People's Republic of China
Tomato canker quarantine and identification methods
michiganensis (Smith) Davisetal.
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, South China Agricultural University, China Agricultural University, Chinese people
Republic of Beijing Exit Inspection and Quarantine.
The main drafters of this standard. Le ocean, Peng Ren, Liu Qiongguang, Luo Xin, Zhang Jianjun, Liu Yong, Feng Yan, Zhong Gong years, Lin Youwei, Zhang Weidong,
Gao Na.
Tomato canker quarantine and identification methods
1 Scope
This standard specifies the tomato canker quarantine authentication methods.
This standard applies to tomato seeds, seedlings and other material related Solanaceae tomato canker quarantine and identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 tomato canker basic information
Chinese name. Tomato canker (rod-shaped bacilli Michigan Michigan subspecies).
Scientific name. Clavibactermichiganensissubsp.michiganensis (Smith, 1910) Davisetal, 1984, referred to.
Cmm).
Synonyms. CorynebacteriummichiganensepV.michiganense (Smith) Dye
michiganense (Smith) Jensen.
Disease English. Bacterialcankeroftomato.
Spp domain Bacteria, Actinobacteria Actinobacteria, actinomycetes Actinobacteria, Actinomycetales Actinomvcetales,
Microbacterium Branch Microbacteriaceae, Rod genus Clavibacter.
Long-distance spread of the pathogen mainly by contaminated seed, tomato seed inner and outer layers can be contaminated. In the field and greenhouse, the bacteria from injury
Mouth, porosity and natural orifice invade host tissue, mainly through irrigation water, rain water, pruning shears, training materials and other communication. The bacteria in the soil and disease
Debris organization can survive for 2 years to 3 years.
Other basic information, see Appendix A. The tomato canker
4 principle of the method
According hazards like tomato canker, the disease symptoms of suspected disease quarantine site or field survey found that samples were collected and
Back to the lab for further isolation and identification, according to the culture traits pathogens, pathogenic response and molecular characterization of the species were
determination.
5 apparatus and appliances
Biological microscope, clean benches, Autoclave, light incubator, temperature oscillation incubator, electronic scales, deep freezer, then
Three Ring, toothpicks, syringes, heated water bath, high-speed refrigerated centrifuge (12000r/min or more), a homogenizer, PCR instrument, electrophoresis apparatus (electric
Swimming instrument and horizontal electrophoresis tank), gel imaging analyzer, micro adjustable pipette and the like.
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