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GB/T 21916-2008 English PDF

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GB/T 21916-2008: Determination of synthetic colour in fruit tins -- High performance liquid chromatography
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GB/T 21916-2008English169 Add to Cart 3 days [Need to translate] Determination of synthetic colour in fruit tins -- High performance liquid chromatography Valid GB/T 21916-2008

PDF similar to GB/T 21916-2008


Standard similar to GB/T 21916-2008

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Basic data

Standard ID GB/T 21916-2008 (GB/T21916-2008)
Description (Translated English) Determination of synthetic colour in fruit tins -- High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X74
Classification of International Standard 67.080
Word Count Estimation 7,745
Date of Issue 2008-05-16
Date of Implementation 2008-11-01
Regulation (derived from) National Standard Approval Announcement 2008 No.9 (Total No.122)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the canned fruit in lemon yellow, amaranth, indigo, carmine, sunset yellow, Allura Red, bright blue, Erythrosin HPLC method of synthetic colorants. This standard applies to canned fruit in lemon yellow, amaranth, indigo, carmine, sunset yellow, Allura Red, bright blue, determination Erythrosin synthetic colorants, detection limit of the standard lemon 0. 300 mg/kg, amaranth 0. 300 mg/kg, indigo 0. 300 mg/kg, Carmine 0. 300 mg/kg, sunset yellow 0. 150 mg/kg, Allura Red 0. 150 mg/kg, bright blue 0. 100 mg/kg, Erythrosin 0. 150 mg/kg.

GB/T 21916-2008: Determination of synthetic colour in fruit tins -- High performance liquid chromatography


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of synthetic colour in fruit tins. High performance liquid chromatography ICS 67.080 X74 National Standards of People's Republic of China Determination of canned fruits synthesized colorant High Performance Liquid Chromatography Posted 2008-05-16 2008-11-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the national food safety emergency standardization working group proposed and managed. This standard was drafted. Shenyang Product Quality Supervision and Inspection Centre (Country processed foods and additives Quality Supervision and Inspection Center). The main drafters of this standard. to stand strong, Dong Wang Yan, Su Xihui, Zhaoli Xiu, Lu Cheng-Xue, Zhang Fengqing, seized Zhao, Zhang Lijun, Huang Shijun, Ming Li Yan, Yang Xiaoli. Determination of canned fruits synthesized colorant High Performance Liquid Chromatography

1 Scope

This standard specifies the canned fruit in lemon yellow, amaranth, indigo carmine, sunset yellow, Allura Red, Brilliant Blue, erythrosine synthetic forward HPLC method for the toner. This standard applies to canned fruit in lemon yellow, amaranth, indigo carmine, sunset yellow, Allura Red, Brilliant Blue, erythrosine synthetic forward Determination of toner. The standard detection limit of lemon 0.300mg/kg, amaranth 0.300mg/kg, indigo 0.300mg/kg, Carmine 0.300mg/kg, Sunset yellow 0.150mg/kg, the temptation to red 0.150mg/kg, light blue 0.100mg/kg, erythrosine 0.150mg/kg. Principle 2 Canned fruit in synthetic colorants with ethanol - ammonia extraction, solid phase extraction (SPE), high performance liquid chromatography - diode Array detector measurement, external standard.

3 Reagents and materials

3.1 Water. deionized water or equivalent purity. 3.2 Methanol. chromatographically pure. 3.3 ethanol. AR. 3.4 ammonia. AR. 3.5 hydrochloric acid. AR. 3.6 ammonium acetate solution (0.02mol/L). Weigh 1.54g ammonium acetate, dissolved in water, dilute to 1000mL, through 0.45μm filter filter. 3.7 ethanol - ammonia solution. the amount of anhydrous ethanol 80mL, ammonia 1mL, add water to 100mL, and mix. 50% 3.8 pH8 methanol. amount of methanol 50mL, add water to 100mL, mix, add ammonia to adjust the pH value to 8. 3.9 pH8 water. water plus ammonia to adjust the pH value to 8. 3.10 2% aqueous ammonia. amount of ammonia 2mL, add water to 100mL, and mix. 3.11 hydrochloric acid - ethanol solution (1 + 9). amount of hydrochloric acid 10mL, 90mL ethanol, and mix. 3.12 50% methanol solution. amount of methanol 50mL, add water to 100mL, and mix. 3.13 SPE or equivalent by. Sep-PakPlusQMA360mg, particle size of 37μm ~ 55μm, followed by adding 5mL before use Methanol (3.2) and 5mL water pretreatment column kept moist. 3.14 synthetic colorants standard stock solution. Weigh accurately translated its purity was 100% by mass of lemon yellow, amaranth, indigo, carmine, Sunset yellow, Allura Red, Brilliant Blue, erythrosine each 0.0500g, set 100mL flask, add water to the mark, formulated as 500μg/mL standard Stock solution. 3.15 synthetic colorants standard solution. When you use the stock standard solution was diluted with water into a standard intermediate solution 20μg/mL, and then the middle It was diluted into a series of standard solution 0.100,0.200,0.400,1.00,3.00,5.00μg/mL, dried 0.45μm filter.

4 Instrument

4.1 high performance liquid chromatography with diode array detector.

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