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SN/T 4076-2014: Detection and identification of lily symptomless virus
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Detection and identification of lily symptomless virus
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SN/T 4076-2014
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Basic data | Standard ID | SN/T 4076-2014 (SN/T4076-2014) | | Description (Translated English) | Detection and identification of lily symptomless virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 16,137 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | SN/T 2119; SN/T 2122 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the lily symptomless virus immunology, molecular biology quarantine and identification methods. This standard applies to the lily family, Inca lily, tulip, bulb scales Kelpie banana spp host plants, quarantine and identification le | SN/T 4076-2014: Detection and identification of lily symptomless virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Lily symptomless virus quarantine and identification methods)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Lily symptomless virus quarantine and identification methods
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Fujian Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Liao Furong, Shen Jianguo, Fang Zhipeng, Lin Shiming, Wu Yuan, Chen Qing, Chen Yun, Peng Ying Huang.
Lily symptomless virus quarantine and identification methods
1 Scope
This standard specifies the lily symptomless virus immunology, molecular biology quarantine and identification methods.
This standard applies to the lily family, the bulb scales Inca lily, tulip, H. littoralis and other host plants of the genus, the leaves of lily symptomless virus
Quarantine identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2119 entry quarantine procedures ornamental bulb scales
SN/T 2122 entry and exit quarantine of plants and plant products sampled
3 Lily symptomless virus
English name. lilysymptomlessvirus
Abbreviation. LSV
Synonyms. lilystreakvirus, lilysymptomlesscarlavirus, lilycurlstripevirus, Alstroemeriacarla-
virus, Alstroemerialatentvirus, lilyvirus.
Taxonomic position. turnip yellow mosaic virus mesh (Tymovirales), β linear virus family (Betaflexiviridae), carnation latent virus genus
(Carlavirus).
Transmission. non-persistent spread by aphids, mechanical dissemination and propagation of the ball.
LSV For additional information, see Appendix A.
4 principle of the method
Lily symptomless virus (LSV) in immunology, molecular characterization is the main basis for the development of methods for identification of the quarantine.
5 major equipment and appliances
Plant juice extractor, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR instrument, electrophoresis, horizontal electrophoresis tank gel into
Imager, high-speed refrigerated centrifuge desktop, thermostat water bath or incubator, pH meter, a variety of range adjustable pipette (1000μL, 200μL,
100μL, 20μL, 10μL, 2μL).
6 Reagents
Serological reagents see Appendix B, molecular biology reagents, see Appendices C and D.
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