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SN/T 2101-2016: Determination of Mycobacterium tuberculosis in milk and dairy products for export - Real-time PCR method
Status: Valid SN/T 2101: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2101-2016 | English | 229 |
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Determination of Mycobacterium tuberculosis in milk and dairy products for export - Real-time PCR method
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SN/T 2101-2016
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| SN/T 2101-2008 | English | 319 |
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Determination of Mycobacterium tuberculosis for milk and milk products. Real-time PCR method
| Obsolete |
SN/T 2101-2008
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PDF similar to SN/T 2101-2016
Basic data | Standard ID | SN/T 2101-2016 (SN/T2101-2016) | | Description (Translated English) | Determination of Mycobacterium tuberculosis in milk and dairy products for export - Real-time PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 10,128 | | Date of Issue | 3/9/2016 | | Date of Implementation | 2016-10-01 | | Older Standard (superseded by this standard) | SN/T 2101-2008 | | Regulation (derived from) | [2016] 131 State Quality Inspection | | Issuing agency(ies) | General Administration of Customs | SN/T 2101-2008: Determination of Mycobacterium tuberculosis for milk and milk products. Real-time PCR method
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Determination of Mycobacterium tuberculosis for milk and milk products.Real-time PCR method
Exit inspection and quarantine industry standard book People's Republic of China
Milk and dairy products Mycobacterium tuberculosis detection methods in
Fluorescence quantitative PCR
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Woo, Huchuan Wei, Jia, Li Ye, LI Zhen-Rong, Sun Yingjie?.
This is the first issue of inspection and quarantine industry standards.
Milk and dairy products Mycobacterium tuberculosis detection methods in
Fluorescence quantitative PCR
1 Scope
This standard specifies the type of milk and dairy products of bovine tuberculosis and human-type operation method of Mycobacterium tuberculosis PCR for detection.
This standard applies to milk, milk powder Mycobacterium tuberculosis detection, other dairy products may refer to.
Principle 2
Milk and Mycobacterium tuberculosis PCR assay method is the use of dairy products in the classic TaqMan probe technology. According to bovine and human
Conserved sequence of Mycobacterium tuberculosis to design a pair of primers and a dual-labeled probes specific fluorescent probes 5 'and 3' end points
Do not use FAM and TAMRA fluorescence-labeled. When the PCR reaction annealing, the primers and probes simultaneously with the target gene fragment binding at this time
Report on the probe fluorophore FAM fluorescence signal emitted by the fluorophore is quenched by TAMRA absorption instrument undetectable FAM group
Mission fluorescence signal emitted; when the PCR reaction is carried out to extend, T Rao q enzyme under the guidance of primer to four nucleotides as a substrate, according to the base
Base pairing principle, the synthesis of new strand along the template strand, when carried to the probe binding sites, the hindered probes can not continue at this time
T Rao q enzyme plays its 5 '→ 3' exonuclease function of the probe into the single nucleotide extension in order to continue to complete the process, FAM group
And TAMRA groups are free in solution, the instrument can detect the fluorescence signal emitted by FAM group.
3 Abbreviations
The following abbreviations apply to this standard.
3.1
Fluorescent PCR
Fluorescent polymerase chain reaction.
3.2
When the number of cycles of each reaction tube fluorescent signal reaches the set value illustrates experienced.
3.3
DNA
DNA.
3.4
T Rao enzyme q
T Rao q DNA polymerase.
4 Materials and Reagents
4.1 Instruments and Equipment
4.1.1 PCR fluorescence detector.
4.1.2 high-speed desktop refrigerated centrifuge.
4.1.3 ordinary tabletop centrifuge.
4.1.4 Vortex.
4.1.5 Refrigerator. 4 ℃ ± 1 ℃, -20 ℃ ± 2 ℃.
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