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SN/T 1151.4-2011: Quarantine protocol for shrimp yellow head disease
Status: Valid SN/T 1151.4: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1151.4-2011 | English | 319 |
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Quarantine protocol for shrimp yellow head disease
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SN/T 1151.4-2011
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| SN/T 1151.4-2003 | English | 359 |
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Protocol of reverse transcription polymerase chain reaction (RT-PCR) for yellowhead virus of shrimp (YHV)
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SN/T 1151.4-2003
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PDF similar to SN/T 1151.4-2011
Basic data | Standard ID | SN/T 1151.4-2011 (SN/T1151.4-2011) | | Description (Translated English) | Quarantine protocol for shrimp yellow head disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 12,196 | | Date of Issue | 2011-05-31 | | Date of Implementation | 2011-12-01 | | Older Standard (superseded by this standard) | SN/T 1151.4-2003 | | Quoted Standard | GB/T 18088 | | Adopted Standard | OIE Aquatic Animal Disease Diagnostic Manual 2009, MOD | | Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 291 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of operation of shrimp yellow head disease RT-PCR and Real-time RT-PCR detection. This section applies to shrimp yellow head disease epidemiology, diagnosis, quarantine and monitoring. | SN/T 1151.4-2011: Quarantine protocol for shrimp yellow head disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for shrimp yellow head disease
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1151.4-2003
Shrimp yellow head disease quarantine Technical Specifications
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1151 series of standards is divided into six parts.
--- Taura Syndrome shrimp quarantine technical specifications;
--- White spot disease quarantine technical specifications;
--- Monodon baculovirus (MBV) diagnostic methods;
--- Shrimp yellow head disease quarantine technical specifications;
--- Shrimp baculovirus (BP) test methods;
--- White Spot virus dot blot and in situ hybridization procedures.
This section SN/T 1151 Part 4.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The partial replacement of SN/T 1151.4-2003 "shrimp yellow head virus (YHV) reverse transcription polymerase chain reaction (RT-PCR) detection side
law".
This section compared with the SN/T 1151.4, the main technical changes are as follows.
--- Add nested RT-PCR method for the differential diagnosis;
--- New Real-timeRT-PCR detection method.
The partial modification using World Organisation for Animal Health (OIE) of "aquatic animal disease diagnostic manual" (2009 edition) 2.2.7 chapter.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shenzhen People's Republic of China Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this section. Xiong Wei, Jiang Jing, Zhang Qiang, Liu Orientin, Li Jian, Wang Qiao whole, Qiu Lu, Huang Zhongrong, Hu Yongqiang.
This part of the standard replaces the previous editions are.
--- SN/T 1151.4-2003.
Shrimp yellow head disease quarantine Technical Specifications
1 Scope
SN/T 1151 This section specifies the method of operation of shrimp yellow head disease RT-PCR and Real-timeRT-PCR detection.
This section applies to shrimp yellow head disease epidemiology, diagnosis, quarantine and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Overview
Shrimp yellow head disease (Yelowheaddisease, YHD) is a shrimp by the yellow head virus (Yelowheadvirus, YHV) caused serious
Threat of shrimp diseases, widespread infection, death rate, the World Organisation for Animal Health (OIE) prescribed notifiable aquatic animal diseases.
Currently, only South Penaeus vannamei outbreaks YHD outbreaks reported, but the Japanese shrimp, white shrimp, krill, also can
Naturally infected with YHV. YHV belong to the yellow head syndrome virus (Yelowheadcomplexofviruses), the group currently has six virus
Known genotype, YHV (genotype 1) is one of a type, and is the only pathogen yellow head disease. Gill associated virus (Gil-
associatedvirus, GAV) belongs to genotype 2. GAV and several other type (type genotype 3 to 6) in East Africa, Asia and Australia
Health monodon are more common, but rarely cause disease. YHV is enveloped baculovirus whose genome is a single positive-stranded RNA.
4 Reagents and materials
4.1 Taq Taq DNA polymerase enzyme 10-fold reaction buffer. Taq enzyme concentration 5U/μL, -20 ℃, avoid repeated freezing and thawing.
4.2 10 times reverse transcriptase enzyme reverse transcriptase reaction buffer. reverse transcriptase concentration of 50U/μL, -20 ℃, avoid repeated freezing and thawing.
4.3 RNA inhibitor (40U/μL). - 20 ℃, avoid repeated freezing and thawing.
4.4 dNTP. containing dATP, dGTP, dCTP, dTTP each 10mmol/L, -20 ℃ preservation.
4.5 RT-PCR primers were. concentration 20μmol/L, which sequence is as follows.
Primer (10F). 5'-CCG-CTA-ATT-TCA-AAA-ACT-ACG-3 ';
Primer (144R). 5'-AAG-GTG-TTA-TGT-CGA-GGA-AGT-3 ';
Primer (GY1). 5'-GAC-ATC-ACT-CCA-GAC-AAC-ATC-TG-3 ';
Primer (GY2). 5'-CAT-CTG-TCC-AGA-AGG-CGT-CTA-TGA-3 ';
Primer (GY4). 5'-GTG-AAG-TCC-ATG-TGT-GTG-AGA-CG-3 ';
Primer (GY5). 5'-GAG-CTG-GAA-TTC-AGT-GAG-AGA-ACA-3 ';
Primer (Y3). 5'-ACG-CTC-TGT-GAC-AAG-CAT-GAA-GTT-3 ';
Primer (G6). 5'-GTA-GTA-GAG-ACG-AGT-GAC-ACC-TAT-3 ';
Primer (YC-F1ab). 5'-ATC-GTC-GTC-AGC-TAC-CGC-AAT-ACT-GC-3 'and
5'-ATC-GTC-GTC-AGY-TAY-CGT-AAC-ACC-GC-3 'mixing the two primers;
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