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NY/T 725-2003: Determination of monensin in feeds. High performance liquid chromatography
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| NY/T 725-2003 | English | 179 |
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Determination of monensin in feeds. High performance liquid chromatography
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NY/T 725-2003
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Basic data | Standard ID | NY/T 725-2003 (NY/T725-2003) | | Description (Translated English) | Determination of monensin in feeds. High performance liquid chromatography | | Sector / Industry | Agriculture Industry Standard (Recommended) | | Classification of Chinese Standard | B20 | | Classification of International Standard | 65.120 | | Word Count Estimation | 5,580 | | Date of Issue | 2003-12-01 | | Date of Implementation | 2004-03-01 | | Summary | This standard specifies the detection of monensin in animal feed by HPLC (HPLC) method. This standard applies to feed, concentrate feed and premix feed additives monensin content. The method detection limit of 5mg/kg. | NY/T 725-2003: Determination of monensin in feeds. High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of monensin in feeds.High performance liquid chromatography
ICS 65.120
B20
People's Republic of China Agricultural Industry Standard
Determination of monensin in feed
High performance liquid chromatography
Released on December 12,.2003
2004-03-01 implementation
Published by the Ministry of Agriculture
Foreword
This standard is based on the general analysis methods recommended by the National Feed Association (NFIA) and a large number of domestic and foreign literature.
According to the research of China's technological development level, high performance liquid chromatography (HPLC)-post-column derivatization was adopted.
This standard was proposed by the Ministry of Agriculture of the People's Republic of China and the National Feed Industry Standardization Technical Committee.
This standard is under the jurisdiction of the National Feed Industry Standardization Technical Committee.
This standard was drafted. National Veterinary Drug Evaluation Center (National Agricultural University Animal Medical College).
The main drafters of this standard. Yu Yuming, Shen Jianzhong, Zhang Suxia, Yuan Jianmin.
Determination of monensin in feed
High performance liquid chromatography
1 Scope
This standard specifies a high performance liquid chromatography (HPLC) method for the detection of monensin in animal feed.
This standard applies to the determination of monensin in compound feed, concentrated feed and additive premix feed. The detection limit of this method is
5mg/kg.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (excluding errata content) or modified versions are not suitable for this standard. However, it is encouraged whether the parties that have reached an agreement under this standard may
Use the latest version of these files. For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 14699.1 Feed sampling method
3 Principle of the method
Extracting monensin from the sample with methanol-water, using methanol-glacial acetic acid-water as the mobile phase, vanillin as the derivatizing agent, and using high-performance liquid color
Spectro-post-column derivatization-ultraviolet detection separation assay.
4 reagents and solutions
Unless otherwise stated, the reagents used in this method are of analytical grade and the water is deionized water, which meets the requirements of GB/T 6682 secondary water.
4.1 Sample extract. [methanol (chromatographic purity) + water] (90 + 10).
4.2 Vanillin Derivatization Solution. Measure 5 mL of concentrated sulfuric acid, slowly add to 250 mL of methanol, place in an ice water bath, and slowly add 20 g of vanilla.
Aldehyde, mix, de-gas, save in the dark, ready to use.
4.3 Monensin standard solution.
4.3.1 Monensin standard stock solution. Accurately weigh the monensin standard (purity ≥90%) 0.1000g, placed in a 100mL volumetric flask
In the solution, the solution was dissolved in methanol, and the concentration was 1000 μg/mL, and stored in a refrigerator at 4 ° C.
4.3.2 Monensin standard working solution. accurately draw a certain amount of standard stock solution (4.4.1), placed in a 10mL volumetric flask, with a
The alcohol was diluted and fixed to a concentration of 2.0 μg/mL, 4.0 μg/mL, 6.0 μg/mL, 8.0 μg/mL, 10.0 μg/mL, and 12.0 μg/mL.
Standard working fluid.
5 Instruments and equipment
Instruments and equipment commonly used in laboratories.
5.1 High Performance Liquid Chromatograph. Post-column derivatization unit and UV detector.
5.2 Centrifuge.
5.3 Oscillator.
5.4 Glass stoppered triangular flask (250mL).
5.5 Microsampler.
5.6 Microporous membrane (0.45 μm).
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