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GB/T 38165-2019 English PDF

Standard Briefing:

Stadard ID: GB/T 38165-2019
Stadard Title: Detection of circulating cell-free DNA concentration in human peripheral blood - Real-time PCR method based on Alu sequence
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GB/T 38165-2019English199 Add to Cart 3 days [Need to translate] Detection of circulating cell-free DNA concentration in human peripheral blood - Real-time PCR method based on Alu sequence Valid GB/T 38165-2019

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Basic Data:

Standard ID GB/T 38165-2019 (GB/T38165-2019)
Description (Translated English) Detection of circulating cell-free DNA concentration in human peripheral blood -- Real-time PCR method based on Alu sequence
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A40
Classification of International Standard 07.080
Word Count Estimation 10,165
Date of Issue 2019-10-18
Date of Implementation 2019-10-18
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

Similar Standards:

GB/T 39995   GB/T 40173   GB/T 38505   GB/T 38164   GB/T 38163   

GB/T 38165-2019: Detection of circulating cell-free DNA concentration in human peripheral blood - Real-time PCR method based on Alu sequence


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Detection of circulating cell-free DNA concentration in human peripheral blood--Real-time PCR method based on Alu sequence ICS 07.080 A40 National Standards of People's Republic of China Detection of circulating free DNA concentration in human peripheral blood Real-time fluorescence PCR based on Alu sequence Published on.2019-10-18 2019-10-18 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Biochemical Testing Standardization Technical Committee (SAC/TC387). This standard was drafted. Chengdu University of Traditional Chinese Medicine, Sichuan Cancer Hospital, Shanghai Institute of Metrology and Testing Technology. The main drafters of this standard. He Yang, Xiao Hongtao, Song Yi, Li Yan, Wen Yanli, Leng Ping, Liu Gang, Li Dongmei. Detection of circulating free DNA concentration in human peripheral blood Real-time fluorescence PCR based on Alu sequence

1 Scope

This standard specifies the real-time fluorescent polymerase chain of circulating free DNA (DNA) based on Alu sequence in human peripheral blood. Reaction (PCR) quantitative detection method. This standard applies to the quantitative detection of Alu sequences in circulating free DNA samples.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosafety

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Circulating free DNA circulatingcel-free DNA; cfDNA A short fragment of DNA in the extracellular free state derived from apoptosis and necrosis in the blood. 3.2 Real-time fluorescent PCR real-time PCR Adding a fluorophore to the PCR reaction system, monitoring the entire PCR amplification process in real time through the accumulation of fluorescent signals, and achieving the initial Quantitative and qualitative analysis of the template. 3.3 Ct value cyclethreshold The number of cycles experienced by the fluorescent signal in each reaction tube reaching a set threshold. 3.4 Alu sequence Alusequence A moderately repeating sequence scattered throughout the primate genome. Note. Alu sequences have 70% to 98% homology, accounting for 5% to 10% of the human genome sequence, and each element is approximately 300 bp in length.

4 Principle

The extracted peripheral blood circulating free DNA is used as a detection target sample, and the qualified human genomic DNA quantitative standard substance is Standards, using real-time fluorescent PCR technology, design specific primers to amplify fragments of 115 bp and 219 bp in Alu sequences, respectively. In the paragraph, the logarithm of the DNA concentration of the standard starting template is the abscissa and the Ct value is the ordinate, and a standard curve is prepared. According to the standard curve equation The Alu sequence copy number in the sample was calculated and the results are expressed in copies/μL.