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GB/T 38164-2019 English PDF

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GB/T 38164-2019: Identification of animal ingredient from common livestock and poultry - Real-time PCR
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Basic data

Standard ID GB/T 38164-2019 (GB/T38164-2019)
Description (Translated English) Identification of animal ingredient from common livestock and poultry - Real-time PCR
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A40
Classification of International Standard 07.080
Word Count Estimation 14,112
Date of Issue 2019-10-18
Date of Implementation 2019-10-18
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 38164-2019: Identification of animal ingredient from common livestock and poultry - Real-time PCR


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Identification of animal ingredient from common livestock and poultry--Real-time PCR ICS 07.080 A40 National Standards of People's Republic of China Common livestock and poultry animal-derived components detection method Real-time fluorescent PCR Real-timePCR Published on.2019-10-18 2019-10-18 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Biochemical Testing Standardization Technical Committee (SAC/TC387). This standard was drafted. China Academy of Inspection and Quarantine, Dalian Customs, Qingdao Customs, Shanghai Customs, Shenyang Customs. The main drafters of this standard. Chen Ying, Han Jianxun, Zheng Qiuyue, Gao Hongwei, Zhang Shuya, Wang Wei, Sun Min, Xing Wei, Zhang Jiukai, Deng Tingting, Wang Jinling, Xu Junyi. Common livestock and poultry animal-derived components detection method Real-time fluorescent PCR

1 Scope

This standard specifies the real-time fluorescent PCR detection method for common animal and animal components. This standard applies to meat and processed products, internal organs, milk, animal feed, yellow cattle, yak, buffalo, sheep, goats, pigs, camels, red deer, plum blossoms Real-time fluorescence of Taqman probes for deer, reindeer, rabbit, dog, chicken, duck, goose, donkey, pigeon, turkey, cat, fox, otter, cockroach, and rat species Qualitative detection by PCR. Detection limit (LOD) 1% (mass score).

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 27403-2008 Laboratory quality control regulations Food molecular biology testing GB/T 34796 Determination of the concentration and purity of nucleic acids in aqueous solutions by ultraviolet spectrophotometry GB/T 35918 animal-derived detection gene bar code technology in animal products Sanger sequencing method 3 terms and definitions, abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Real-time fluorescent PCR real-time PCR A fluorophore is added to the PCR reaction system, and the entire PCR amplification process is monitored in real time by accumulation of fluorescent signals. 3.1.2 Ct value cyclethreshold The number of cycles experienced by the fluorescent signal in each reaction tube reaching a set threshold. 3.2 Abbreviations The following abbreviations apply to this document. ATP8. ATP synthase subunit 8 (ATPsynthasesubunit8) COI. Cytochrome C oxidase I CTAB. Cetyltrithylammonium bromide Cytb. cytochrome b (Cytochromeb) DNA. Deoxyribonucleic acid D-loop. D-ring zone (Displacementloopregion) EDTA. Ethylenediaminetetraacetic acid

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