|
US$199.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 36433-2018: Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36433-2018 | English | 199 |
Add to Cart
|
3 days [Need to translate]
|
Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method
| Valid |
GB/T 36433-2018
|
PDF similar to GB/T 36433-2018
Basic data | Standard ID | GB/T 36433-2018 (GB/T36433-2018) | | Description (Translated English) | Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | W04 | | Classification of International Standard | 59.080.01 | | Word Count Estimation | 10,179 | | Date of Issue | 2018-06-07 | | Date of Implementation | 2019-01-01 | | Regulation (derived from) | National Standards Announcement No. 9 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36433-2018: Textiles -- DNA quantitative analysis of cashmere and wool mixture -- Fluorescence PCR method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Textiles--DNA quantitative analysis of cashmere and wool mixture--Fluorescence PCR method
ICS 59.080.01
W04
National Standards of People's Republic of China
Textile cashmere and sheep wool
Quantitative analysis of mixture DNA by fluorescence PCR
Published on.2018-06-07
2019-01-01 Implementation
National Market Supervision Administration
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the China Textile Industry Federation.
This standard is under the jurisdiction of the National Textile Standardization Technical Committee (SAC/TC209).
This standard was drafted by Shanghai Entry-Exit Inspection and Quarantine Bureau and China Textile Standard Inspection & Certification Co., Ltd.
The main drafters of this standard. Fei Jing, Xie Yuman, Rui Yanghua, Si Ying, Tang Minfeng, Lu Weimin.
Textile cashmere and sheep wool
Quantitative analysis of mixture DNA by fluorescence PCR
1 Scope
This standard specifies the quantitative DNA detection method for the determination of cashmere and sheep wool in textiles using fluorescent PCR.
This standard applies to the detection of two components of cashmere and sheep wool in textile blends.
This standard does not apply to recycled, stripped cashmere and sheep wool fibers, and Angora wool.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB/T 16988 Determination of Mixture of Special Animal Fibers and Sheep Wool
General requirements and definitions for GB/T 19495.1 genetically modified products
GB/T 19495.2 Laboratory Testing Requirements for Genetically Modified Products
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Polymerase chain reaction polymerase chain reaction; PCR
Under the catalysis of DNA polymerase, using the parent strand DNA as a template and the specific primers as the starting points, 4 kinds of single nucleotides (dNTP) are
The substrate, through a denaturing, annealing, and extended cyclic reaction, causes a very small amount of specific fragments of DNA to be amplified millions of times in a matter of hours.
In vitro amplification of DNA by chain reaction.
3.2
Real-time fluorescence PCR
Fluorescence groups are added to the PCR reaction system to monitor the entire PCR process in real-time using fluorescence signal accumulation and then pass the curve to unknown
Template for quantitative or qualitative analysis of DNA amplification methods.
3.3
Primer primer
In the nucleic acid synthesis reaction, a small piece of single-stranded DNA that functions as a starting point for the extension of each polynucleotide strand and acts
Or RNA.
3.4
TaqMan probe TaqManprobe
One fluorophore is attached to the 5' end of the probe and the quencher is the 3' end of the oligonucleotide.
Note. A specific fluorescent probe is added at the same time that a pair of primers is added during PCR amplification. When the probe is intact, the fluorescence signal emitted by the reporter group is quenched.
The group absorbs; during PCR amplification, the Taq enzyme's 5′-3′ exonuclease activity degrades the probe, causing the reporter and quencher fluorophores to separate.
The fluorescence monitoring system can receive the fluorescence signal, that is, one fluorescent molecule is formed for each DNA strand to be amplified, and the fluorescence signal is accumulated.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36433-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36433-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 36433-2018_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 36433-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|