GB/T 31270.11-2014 (GB/T31270.11-2014, GBT 31270.11-2014, GBT31270.11-2014) & related versions
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Test guidelines on environmental safety assessment for chemical pesticides -- Part 11: Silkworm acute toxicity test
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GB/T 31270.11-2014
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GBT 31270.11-2014
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GB/T 31270.11-2014
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.100
B 13
Test guidelines on environmental safety assessment
for chemical pesticides - Part 11. Silkworm acute
toxicity test
ISSUED ON. OCTOBER 10, 2014
IMPLEMENTED ON. MARCH 11, 2015
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People's Republic of China;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword ... 3
1 Scope ... 5
2 Terms and definitions ... 5
3 Test summary ... 6
4 Test method ... 7
5 Test report ... 10
Annex A ... 11
Foreword
GB/T 31270 Test guidelines on environmental safety assessment for chemical pesticides
consists of 21 parts as follows.
— Part 1. Transformation in soils;
— Part 2. Hydrolysis;
— Part 3. Phototransformation;
— Part 4. Adsorption/desorption in soils;
— Part 5. Leaching in soil;
— Part 6. Volatility;
— Part 7. Bioconcentration test;
— Part 8. Degradation in water-sediment systems;
— Part 9. Avian acute toxicity test;
— Part 10. Honeybee acute toxicity test;
— Part 11. Silkworm acute toxicity test;
— Part 12. Fish acute toxicity test;
— Part 13. Daphnia sp. acute immobilisation test;
— Part 14. Alga growth inhibition test;
— Part 15. Earthworm acute toxicity test;
— Part 16. Soil microorganism toxicity test;
— Part 17. Trichogramma acute toxicity test;
— Part 18. Amphibian acute toxicity test;
— Part 19. Effects on non-target plants;
— Part 20. Livestock short-term dietary toxicity test;
— Part 21. Macro-crustacean toxicity test.
Test guidelines on environmental safety
assessment for chemical pesticides –
Part 11. Silkworm acute toxicity test
1 Scope
GB/T 31270 of this Part specifies the basic requirements such as material, condition,
operation, quality control, data processing, test report of silkworm acute toxicity test,
measured by leaf dipping method and fumigation method.
This Part applies to silkworm leaf dipping and fumigation toxic test, providing a
reference for other types of pesticide.
This Part does not apply to volatile and insoluble chemical pesticides.
2 Terms and definitions
For the purpose of this Part, the following terms and definitions shall apply.
2.1 Medium lethal concentration
In leaf dipping method test, test substance concentration that causes 50% death of test
organism, indicated as LC50.
Note. unit as mg a.i./L.
2.2 Test substance
Substance that needs to be tested in test.
2.3 Chemical pesticide
Pesticide is an artificial pesticide by using chemical substances, in which biomimetic
synthesis pesticide is made by taking active substance of natural products as matrix,
conducting the imitation, structure-change and innovation.
Synonymy. Synthetic organic pesticide
[NY/T 1667.1 -2008, definition 2.3.1]
2.4 Technical material
In the process of manufacturing, the final product that is obtained by combination of
effective elements and impurities. It shall not include visual foreign matter and any
additives; if necessary, a small number of stabilizer shall be added.
[NY/T 1667.2-2008, definition 2.5.1]
2.5 Formulation products
Products with stable service condition that are made from pesticide, original medicine and
additive.
[NY/T 1667.2-2008, definition 2.4]
2.6 Active ingredient. a. i.
Active ingredient refers to special chemical structural ingredient with biological activity.
[NY/T 1667.2-2008, definition 3.1]
2.7 Reference substances
Chemical substance or mixture that is used to prove or deny certain property of test
substance or judge effectiveness of test system.
3 Test summary
3.1 Method summary
Silkworm acute toxicity test includes leaf dipping toxic method and fumigation method.
Choose relevant methods to implement test according to pesticide registration
management rules and other provisions.
3.2 Leaf dipping toxic test
Adopt the solution with different concentrations to dip folium mori; after drying, it shall be
used to feed silkworm. During the whole process of feeding and treatment mori, toxic
symptom and death status of silkworm in test shall be observed after 24 h, 48 h, 72 h and
96 h. After test finishes, analyze the data and calculate medium lethal concentration LC50.
3.3 Fumigation toxic test
Fumigation toxic test is a test, based on health, using medicine to imitate indoor drug
delivery condition; it shall be implemented in fumigation room or fumigation test device in
accordance with test requirements. On the basis of meeting test requirements, relevant
Annex A
(Informative)
Division of toxic level of pesticide to silkworm
Acute toxicity of pesticide can be divided into four levels according to toxic medium lethal
concentration LC50 (96h), by acute leaf dipping method, as shown in Table A.1
Table A.1 Division of toxic level of pesticide to silkworm
Level of toxicity LC50 (96 h)/(mg a. i. /L)
Severe toxic LC50 ≤ 0.5
Highly toxic 0.5 < LC50 ≤ 20
Medium Toxic 20 < LC50 ≤ 200
low toxic LC50 > 200
Fumigation toxic test is a test, based on health, using medicine to imitate indoor drug
delivery condition. If death rate of silkworm is above 10%, then it is deemed that it is high
risk to silkworm.
......
| Standard ID | GB/T 31270.11-2014 (GB/T31270.11-2014) | | Description (Translated English) | Test guidelines on environmental safety assessment for chemical pesticides. Part 11: Silkworm acute toxicity test | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B13 | | Classification of International Standard | 65.100 | | Word Count Estimation | 9,931 | | Date of Issue | 2014/10/10 | | Date of Implementation | 2015/3/11 | | Drafting Organization | Ministry of Agriculture Pesticide Inspection Center | | Administrative Organization | Ministry of Agriculture | | Regulation (derived from) | People's Republic of China Announcement of Newly Approved National Standards 2014 No. 24 | | Proposing organization | Ministry of Agriculture of the People's Republic of China | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies leaf dipping method for the determination of chemical pesticides and fumigation of the basic requirements for material silkworm acute toxicity test, conditions, operations, quality control, data processing, test reports and the lik |
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