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GB/T 23192-2008: Method for determination of starch grains in honey -- Count with microscope
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 23192-2008 | English | 139 |
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Method for determination of starch grains in honey -- Count with microscope
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GB/T 23192-2008
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Basic data | Standard ID | GB/T 23192-2008 (GB/T23192-2008) | | Description (Translated English) | Method for determination of starch grains in honey -- Count with microscope | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X31 | | Classification of International Standard | 67.180.10 | | Word Count Estimation | 6,649 | | Date of Issue | 2008-12-31 | | Date of Implementation | 2009-06-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.27 (Total No.140) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for the determination of starch granules in honey. This standard applies to the determination of qualitative and counting honey starch grains. This standard does not apply to honey products. | GB/T 23192-2008: Method for determination of starch grains in honey -- Count with microscope---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for determination of starch grains in honey. Count with microscope
ICS 67.180.10
X31
National Standards of People's Republic of China
Honey Determination of starch grains
Microscope counting method
Posted 2008-12-31
2009-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard is proposed and managed by China Federation of Supply and Marketing Cooperatives.
This standard was drafted. Bee Beijing Institute of Science and Technology Development Co., Ltd. Beijing flowers Bee Products.
The main drafters of this standard. Malan Yu, Chen Xiaoxia, Guo Lijun.
Introduction
The standard equipment requiring a simple, easy operation, easy to spread. Applicable to all grass-roots business organization's quality control needs.
Honey Determination of starch grains
Microscope counting method
1 Scope
This standard specifies the method for the determination of starch granules in honey.
This standard applies to the determination of qualitative and honey counts of starch grains.
This standard does not apply to honey products.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
Principle 3
Honey sample starch granules may be combined with iodine to generate a blue complex, it can be observed under a microscope count.
When the starch amylose and amylopectin ratio is not at the same time, the colors may appear to be blue, purple or red.
4 Reagents and materials
Unless otherwise indicated, at least in the analysis using analytical reagent. The water is distilled or deionized water, which should be consistent with GB/T 6682 in
Three water standards.
4.1 iodine - potassium iodide solution (0.3%). weigh 3g iodine, 6g potassium iodide, dissolved, and water volume to 1000mL.
4.2 hemacytometer (25 × 16).
4.3 conical graduated centrifuge tube (15mL). minimum scale 0.1mL.
4.4 straw (10mL).
4.5 dropper.
4.6 coverslip.
5 Equipment
5.1 centrifuge (3000r/min).
5.2 microscope (× 100, × 400).
5.3 Balances (a sense of volume 0.01g).
Preparation 6 samples
6.1 Weigh homogeneous honey sample 2.0g, add water, 20mL, boiled cooled. This sample solution used for qualitative testing.
6.2 Weigh uniform honey 50.0g, 25mL diluted with water, and mix. This sample solution is used to test the microscope counting.
7 Procedure
7.1 Qualitative Test
Take honey sample solution (6.1) a little, add 2 drops of iodine solution, the same color as the honey were no starch; if blue, green, purple-doped
Starch, red if the added dextrin.
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