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GB/T 14927.2-2008: Laboratory animal -- Immunological marker of inbred mice and rats
Status: Valid GB/T 14927.2: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 14927.2-2008 | English | 209 |
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Laboratory animal -- Immunological marker of inbred mice and rats
| Valid |
GB/T 14927.2-2008
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| GB/T 14927.2-2001 | English | 199 |
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Laboratory animal Genetic monitoring: skin grafting of inbred mice and rats
| Obsolete |
GB/T 14927.2-2001
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| GB/T 14927.2-1994 | English | 199 |
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Method for examination of laboratory animals. Skin grafting of inbred mice and rats
| Obsolete |
GB/T 14927.2-1994
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PDF similar to GB/T 14927.2-2008
Basic data | Standard ID | GB/T 14927.2-2008 (GB/T14927.2-2008) | | Description (Translated English) | Laboratory animal -- Immunological marker of inbred mice and rats | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B44 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 9,928 | | Date of Issue | 2008-12-10 | | Date of Implementation | 2009-03-01 | | Older Standard (superseded by this standard) | GB/T 14927.2-2001 | | Regulation (derived from) | National Standard Approval Announcement 2008 No.19 (Total No.132) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the inbred mice, rat skin grafting method and inbred mouse H-2 haplotype (Haplo-type) detection method. This part of the inbred mouse, rat skin graft law applies inbred mice and rats genetic purity checks and genetic monitoring process of cultivating inbred mice and rats reared in the breeding process. Inbred mouse H-2 haplotype (Haplotype) detection method is suitable for cultivation of inbred mice and propagation of genetic breeding process monitoring, detection of H-2 antigens main complex area D, and K type. | GB/T 14927.2-2008: Laboratory animal -- Immunological marker of inbred mice and rats---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Laboratory animal. Immunological marker of inbred mice and rats
ICS 65.020.30
B44
National Standards of People's Republic of China
Replacing GB/T 14927.2-2001
Experimental animals
Inbred mice, rats immunolabeling assay
Posted 2008-12-10
2009-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
GB/T 14927 is divided into two parts.
--- Part 1 as "experimental animals inbred mice, rats and biochemical assay mark";
--- Part 2 as "experimental animals inbred mice, rats immunolabeling assay."
This section GB/T Part of 214,927.
Since the implementation of this section, instead of GB/T 14927.2-2001 "animal inbred mice, rat skin graft detection
law".
This part of the main technical differences compared with the GB/T 14927.2-2001 as follows.
a) Amendment of experimental animals inbred mice, rat skin transplantation in part;
b) increase the mouse H-2 haplotypes trace cytotoxicity assay;
c) The part of the name was changed to "animal inbred mice, rats immunolabeling assay."
Appendix A of this section is an informative annex.
This part of the National Standardization Technical Committee of experimental animals and focal points.
This part of the National Standardization Technical Committee responsible for drafting the experimental animals.
The main drafters of this section. Xing Ruichang, Liu bicyclic, Mali Ying, Yuebing Fei, Bao Shimin.
This part of the standard replaces the previous editions are.
--- GB/T 14927.2-1994, GB/T 14927.2-2001.
Experimental animals
Inbred mice, rats immunolabeling assay
1 Scope
GB/T 14927 provisions of this part of the inbred mice, rats and skin grafting method inbred mouse H-2 haplotypes (Haplo-
type) detection method.
This section inbred mice, rat skin grafting method for checking the inbred mice and rats genetic purity in breeding process to
And genetic monitoring of inbred mice and rats in the breeding process. Inbred mouse H-2 haplotype (Haplotype) suitable detection method
It used inbred mouse strains and nurturing the breeding process of genetic monitoring, detection of H-2 antigen main complex area of \u200b\u200bArea D and K type.
2 inbred mouse and rat skin graft law
2.1 Technical Principle
Graft can be accepted each other in the same inbred that syngeneic transplant (isograft) is successful.
Graft rejection in mutually different inbred lines, that allograft (alograft) was unsuccessful.
F1 generation of animals can be a parent to accept any tissue grafts, parents can not accept the F1 generation implant.
F1 generation of animals can be accepted after the F2 generation graft generations of animals.
Parental lines can accept certain F2 generations after generations graft animals, but most of exclusion.
This part of the back and tail skin grafting skin transplantation. The same principle of the two methods, and has the same potency standard.
2.2 dorsal skin grafting
2.2.1 Equipment and Materials
2.2.1.1 fixed plate (18cm × 12cm).
2.2.1.2 sodium pentobarbital (medical).
2.2.1.3 medical adhesive plaster.
2.2.1.4 Vaseline.
2.2.1.5 powder sodium penicillin G (80 million U, human or veterinary).
2.2.1.6 3% iodine tampons.
2.2.1.7 75% alcohol cotton ball.
2.2.1.8 ophthalmic scissors.
2.2.1.9 ophthalmic tweezers.
2.2.1.10 disposable syringe (1mL).
2.2.1.11 gauze (cut 40mm long, 25mm wide and several pieces; thick layer of 2 to 3 layers of several pieces, which is coated Vaseline and powder blue
ADM G sodium).
2.2.1.12 made of cotton tampons.
2.2.1.13 surgical equipment placed in an autoclave 121 ℃, 40min autoclaved.
2.2.2 Procedure
2.2.2.1 randomly with sex 4 weeks to 8 weeks 10 animals, animals can be derived from the basis of kinship group or expand the group.
2.2.2.2 each animal were weighed separately numbered weight strain detailed records of the name, sex, date of birth, lineage and other features.
2.2.2.3 with sterile normal saline 0.7% solution of sodium pentobarbital.
2.2.2.4 intraperitoneal injection of 0.7% sodium pentobarbital anesthetized animals. Each 10g body weight of mice injected 0.1mL, rats per 20g
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