GB/T 35803-2018 PDF EnglishUS$150.00 · In stock · Download in 9 seconds
GB/T 35803-2018: Determination of prohibited urocanic acid and its ethyl ester in cosmetics - High performance liquid chromatography Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
Similar standardsGB/T 35803-2018: Determination of prohibited urocanic acid and its ethyl ester in cosmetics - High performance liquid chromatography---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT35803-2018 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 71.100.70 Y 42 Determination of prohibited urocanic acid and its ethyl ester in cosmetics - High performance liquid chromatography Issued on: FEBRUARY 06, 201 Implemented on: SEPTEMBER 01, 2018 Issued by. General Administration of Quality Supervision, Inspection and Quarantine; Standardization Administration of the People's Republic of China. Table of ContentsForeword ... 3 Introduction ... 4 1 Scope ... 5 2 Normative references ... 5 3 Principle ... 5 4 Reagents and materials ... 5 5 Apparatus and equipment ... 6 6 Analysis steps ... 7 7 Result calculation ... 8 8 Detection limit and limit of quantification ... 9 9 Recovery rate and precision ... 9 10 Tolerance ... 9 Annex A (informative) Chinese name, English name, CAS number, molecular formula, relative molecular mass, structural formula of urocanic acid and its ethyl ester ... Error! Bookmark not defined. Annex C (informative) Test of confirmation ... 13 Determination of prohibited urocanic acid and its ethyl ester in cosmetics - High performance liquid chromatography1 ScopeThis Standard specifies determination method of high performance liquid chromatography for prohibited urocanic acid and its ethyl ester in cosmetics. This Standard is applicable to determination of urocanic acid and its ethyl ester in cosmetics such as creams, liquids, lipsticks, loose powders, shampoos. The detection limit of this Standard for urocanic acid and its ethyl ester is 2 mg/kg, and the limit of quantification is 5 mg/kg.2 Normative referencesThe following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 6682, Water for analytical laboratory use - Specification and test methods3 PrincipleAfter the specimen is centrifuged by solvent and is centrifuged, after the supernatant is filtered by nitrogen blowing capacity, use high performance liquid chromatography to determine, use external standard method to quantify, use liquid chromatography-mass spectrometry to confirm.4 Reagents and materialsUnless otherwise stated, the reagents used are analytically pure, the water is of grade one specified in GB/T 6682. 4.1 Methanol. chromatographically pure. 5.5 Ultrasonic cleaner. 5.6 Nitrogen blower. 5.7 Plugged plastic centrifuge tube. 10mL. 5.8 Graduated nitrogen blow tube. 10mL. 5.9 Microporous membrane. 0.45μm, made of PTFE.6 Analysis steps6.1 Sample processing 6.1.1 Cream, liquid, loose powder and shampoo samples Weigh 0.2g (to the nearest of 0.001g) of specimen in a 10mL plugged plastic centrifuge tube (5.7). Add into 10mL of methanol (4.1). Shake well. After performing ultrasonic extraction for 20min, centrifugation at 8000r/min for 15min, transfer the supernatant to the graduated nitrogen blow tube (5.8). Slow the nitrogen blowing to 1mL. Add into 9mL of water. Mix well. It is filtered through a 0.45μm microporous membrane for high performance liquid chromatography. 6.1.2 Lipstick sample Weigh 0.2g (to the nearest of 0.001g) of specimen in a 10mL plugged plastic centrifuge tube (5.7). Add into 0.5mL of tetrahydrofuran (4.2). Then add into 9.5mL of methanol (4.1). Shake well. Performing ultrasonic extraction for 20min, centrifugation at 8000r/min for 15min. Transfer the supernatant to the graduated nitrogen blow tube (5.8). Slow the nitrogen blowing to 1mL. Add into 9mL of water. Mix well. It is filtered through a 0.45μm microporous membrane for high performance liquid chromatography. 6.2 Determination conditions The reference conditions for high performance liquid chromatography are as follows. a) Chromatographic column. Xterra MS C18, 5μm, 250mm × 4.6mm (inner diameter), or the equivalent; b) Flow rate. 1.0mL/min; c) Mobile phase. methanol + 0.005mol/L tetrabutylammonium bromide solution=30+70 (volume ratio); d) Detection wavelength. 285nm;Annex C(informative) Test of confirmation C.1 Liquid chromatography conditions The reference conditions for liquid chromatography determination are as follows. a) Chromatographic column. XBridge C18, 3.5μm, 150mm × 2.1mm (inner diameter); b) Mobile phase. 0.1% formic acid solution + acetonitrile = 95 + 5 (volume ratio); c) Flow rate. 0.3mL/min; d) Column temperature. 30°C; e) Injection volume. 5μL. C.2 Mass spectrometry conditions The reference conditions for mass spectrometry are as follows. a) Ion source. electrospray ion source; b) Ionization mode. positive ion mode; c) Capillary voltage. 3kV; d) Extraction voltage. 2.9V; e) Ion source temperature. 150°C; f) Desolvation gas temperature. 350°C; g) Data collection method. multiple reaction monitoring. C.3 Qualitative determination When the specimen is measured, the sample solution is appropriately diluted, and the sample solution and the standard working solution are determined according to liquid chromatography-mass spectrometry conditions. If the selected ions are present, and the selected ion ratio is consistent with the ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of GB/T 35803-2018 be delivered?Answer: The full copy PDF of English version of GB/T 35803-2018 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of GB/T 35803-2018_English with my colleagues?Answer: Yes. 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